CRCU

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (6): 1031-1034.

• Articles • Previous Articles     Next Articles

Down-regulation of Vinculin in Human Colorectal Carcinoma Identified by Proteomics Analysis

YANG Guang-yuan1,2, ZHAO Hai-feng2, XIE Feng1, LI Yi4, SUN Li3, SHI Qing-hong1, HE Cheng-yan1, LIU Ning3, SONG Li-na1   

  1. 1. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    2. The First Affiliated Hospital of Jiamusi University, Jiamusi 154003, P. R. China;
    3. The Second Hospital of Jilin University, Changchun 130021, P. R. China;
    4. Stomatological School of Jilin University, Changchun 130021, P. R. China
  • Received:2012-03-21 Revised:2012-04-28 Online:2012-11-25 Published:2012-11-09
  • Contact: HE Cheng-yan, LIU Ning E-mail:chengyanhe469@vip.sina.com;liu_ning@jlu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.21107055), the Natural Science Foundation of Jilin Province, China(Nos.200705387, 2011713, 20090461, 20110739) and the Natural Science Foundation of Heilongjiang Province, China(Nos.201000742, D201075, D200873, GC09C317).

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Abstract:

In the present study, we aimed to globally profile the proteins involved in colorectal carcinoma(CRC), in order to find clues to the pathological process of CRC. Pairs of malignant tissues and their adjacent healthy tissues from patients with colorectal cancer were subject to differential proteomics analysis. Two dimensional electrophoresis coupled with mass spectrometry(2-DE/MS) was used to identify differentially expressed proteins between pairs of tissue samples. A list of proteins relevant to the progression of colorectal tumor was identified by two dimensional gel electrophoresis(2-DE)-based proteomics approach. Among the identified proteins, vinculin was found to be remarkably down-regulated in colorectal carcinoma tissues. In addition, three phosphorylation modifications within the isolated vinculin were identified by in-depth liquid chromatography-tandem mass spectrometry(LC-MS/MS) analysis. Our results provide a basis for further understanding the pathological significance of vinculin in the regulation of carcinogenesis, invasion and metastasis of colorectal tumors.

Key words: Mass spectrometry, Vinculin, Colorectal carcinoma, Phosphorylation