Metabonomics and Molecular Biology-based Effects of Sugemule-3 in an Isoproterenol-induced Cardiovascular Disease Rat Model

doi:10.1007/s40242-018-7307-y

Chemical Research in Chinese Universities ›› 2018, Vol. 34 ›› Issue (4): 590-597.doi: 10.1007/s40242-018-7307-y

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Metabonomics and Molecular Biology-based Effects of Sugemule-3 in an Isoproterenol-induced Cardiovascular Disease Rat Model

WANG Xiye¹, WANG Yu², WEI Chengxi², YU Lijun²

1. Inner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, College of Chemistry and Chemical Engineering, Inner Mongolia University for the Nationalities, Tongliao 028005, P. R. China;
2. Inner Mongolia Autonomous Region Key Laboratory of Mongolian Medicine Pharmacology for Cardio-cerebral Vascular System, Medicinal Chemistry and Pharmacology Institute, Inner Mongolia University for the Nationalities, Tongliao 028005, P. R. China

Received:2017-09-18 Online:2018-08-01 Published:2018-06-04
Contact: WEI Chengxi, YU Lijun E-mail:wcx1224@imun.edu.cn;13947550088@163.com
Supported by:
Supported by the National Natural Science Foundation of China(Nos.81560702, 81260682 and 30760299), the Natural Science Foundation of Inner Mongolia, China(Nos.2017BS0809 and 2016BS0806), the School Project of Inner Mongolia University for the Nationalities, China(No.NMDYB1727) and the Open Subject of Inner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, China(No.MDK2017044).

Abstract

Abstract: Cardiovascular disease(CVD) is a common and serious disease in the elderly, which has characteristically high prevalence, disability, and mortality rates. However, the etiology of CVD is still not very clear. The traditional Mongolian medicine Sugemule-3(SM) is usually used for the treatment of CVD and exhibits a good curative effect. In this study, a serum metabolite profile analysis was used to identify potential biomarkers associated with isoproterenol(ISO)-induced CVD and investigate the mechanism of the action of SM. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used for the metabono-mics analysis. Principal component analysis(PCA) was used to process the acquired data to differentiate the results of the control, CVD, and SM treatment groups. Orthogonal partial least squares discriminant analysis(OPLS-DA) enabled the identification of 21 metabolites as potential biomarkers that were relevant to phospholipid and energy metabolism. The results indicate that SM played a protective role against ISO-induced CVD in rats by regulating phospholipid and energy metabolic pathways. Further, we verified the apoptotic metabolic pathway using molecular biology methods, such as terminal deoxynucleotidyl transferase(TdT) deoxyuridine 5'-triphosphate(dUTP) nick-end labeling(TUNEL) assay and Western blot analysis. Furthermore, this study identified early biomarkers of CVD and elucidated the underlying mechanism of the therapeutic actions of SM, which is worth further to be investigated for development as a clinical therapy.

Key words: Sugemule-3, Metabonomics, Molecular biology, Ultra-performance liquid chromatography coupled with mass spectrometry(UPLC-MS), Cardiovascular disease, Isoproterenol

WANG Xiye, WANG Yu, WEI Chengxi, YU Lijun. Metabonomics and Molecular Biology-based Effects of Sugemule-3 in an Isoproterenol-induced Cardiovascular Disease Rat Model[J]. Chemical Research in Chinese Universities, 2018, 34(4): 590-597.

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Metabonomics and Molecular Biology-based Effects of Sugemule-3 in an Isoproterenol-induced Cardiovascular Disease Rat Model

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