Excimer-based Activatable Fluorescent Sensor for Sensitive Detection of Alkaline Phosphatase

doi:10.1007/s40242-021-1194-3

Chemical Research in Chinese Universities ›› 2021, Vol. 37 ›› Issue (4): 960-966.doi: 10.1007/s40242-021-1194-3

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Excimer-based Activatable Fluorescent Sensor for Sensitive Detection of Alkaline Phosphatase

YUAN Fang¹, LI Yang¹, CHEN Zhenjuan², ZHANG Jianjian¹, NING Lulu², YANG Xiao-Feng¹, PU Kanyi^3,4

1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Lab of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, P. R. China;
2. Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, P. R. China;
3. School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore;
4. Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 637371, Singapore

Received:2021-04-29 Revised:2021-06-24 Online:2021-08-01 Published:2021-07-27
Contact: ZHANG Jianjian, NING Lulu, YANG Xiao-Feng, PU Kanyi E-mail:zhangjj@nwu.edu.cn;ninglulu@sust.edu.cn;xfyang@nwu.edu.cn;kypu@ntu.edu.sg
Supported by:
This work was supported by the National Natural Science Foundation of China(Nos.21904105, 22074120), the Fund of Nanyang Technological University, Singapore(No.NTU-SUG:M4081627.120) and the Singapore Ministry of Education, Academic Research Fund Tier 1(Nos.2019-T1-002-045, 2018-T1-001-173) and Academic Research Fund Tier 2(No.MOE2018-T2-2-042).

Abstract

Abstract: The accurate detection of related biomarkers at early stage is crucial in diagnosis and therapy of cancer. Small molecule fluorescence probes stand out acting as efficient tools to detect biomolecules, especially biomacromolecules. Herein, a new probe that features excimer formation has been designed to detect cancer-related biomarker alkaline phosphatase(ALP). The probe shows high sensitivity to ALP with a low limit detection of 0.13 U/L and high selectivity with resistance to the interference in the complex physiological system. Furthermore, this probe presents good biocompatibility with negligible cytotoxicity and displays remarkable cell imaging functions, which allows for its application in biosystems.Theoretical simulation validates the high affinity of the probe with ALP and depicts the details of the interaction modes, which provides the possible mechanisms underlying the efficient detection of the probe in atomic level. The study of synthesis, properties and applications of the activatable emissive excimers not only enriches the tools to detect ALP, but also provides a new strategy to comprehend the pathogenic mechanism of enzyme-related diseases.

Key words: Biosensor, Fluorescence, Excimer, Imaging

YUAN Fang, LI Yang, CHEN Zhenjuan, ZHANG Jianjian, NING Lulu, YANG Xiao-Feng, PU Kanyi. Excimer-based Activatable Fluorescent Sensor for Sensitive Detection of Alkaline Phosphatase[J]. Chemical Research in Chinese Universities, 2021, 37(4): 960-966.

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Excimer-based Activatable Fluorescent Sensor for Sensitive Detection of Alkaline Phosphatase

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