A Long-wavelength Emissive Phenothiazine Derived Fluorescent Probe for Detecting HOCl Upregulation in 5-FU Stimulated Living Cells

doi:10.1007/s40242-021-1173-8

Abstract

Abstract: The endogenous hypochlorous acid(HOCl) has been evidenced in a variety of cellular courses. However, the role of HOCl in most pathophysiological processes still keeps unclear because of the limited detecting tools. In this work, we presented the pre- paration of a phenothiazine-derived fluorescent probe ClO-1 for HOCl detection with a cyanopyridinium moiety to improve its water solubility and lengthen its emission wavelength. The HOCl-promoted oxidation of sulfur atom in the probe resulted in a 460-fold emission intensity enhancement at 635 nm with high selectivity and sensitivity(detection limit: 1.12 nmol/L). The rapid response(5 s) also endowed the probe with real-time detection ability. Successfully, ClO-1 was devoted to the bioimaging of endogenous HOCl in inflamed RAW 264.7 cells and 5-fluorouracil-treated MCF-7 cells.

Key words: Fluorescent probe, Phenothiazine, Hypochlorous acid, 5-Fluorouracil, Bioimaging

WANG Bingya, GUO Xiaomei, LIU Zuodong, WU Yongquan, HOU Ji-Ting. A Long-wavelength Emissive Phenothiazine Derived Fluorescent Probe for Detecting HOCl Upregulation in 5-FU Stimulated Living Cells[J]. Chemical Research in Chinese Universities, 2022, 38(2): 609-615.

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