Chemical Research in Chinese Universities ›› 2020, Vol. 36 ›› Issue (2): 194-202.doi: 10.1007/s40242-019-0031-4

• Reviews • Previous Articles     Next Articles

Fluorescence Signal Amplification Strategies Based on DNA Nanotechnology for miRNA Detection

LI Tao, DUAN Ruilin, DUAN Zhijuan, HUANG Fujian, XIA Fan   

  1. Engineering Research Center of Nano-geomaterials, Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, P. R. China
  • Received:2019-10-30 Revised:2019-11-27 Online:2020-04-01 Published:2020-03-18
  • Contact: HUANG Fujian E-mail:huangfj@cug.edu.cn
  • Supported by:
    Supported by the National Key R&D Program of China(No.2017YFA0208000), the National Natural Science Foundation of China(Nos.21974127, 21525523, 21722507, 21874121, 21974128), and the Natural Science Foundation of Zhejiang Province, China (No.LY20B050001).

Abstract: In this review, the most recent progresses in the field of fluorescence signal amplification strategies based on DNA nanotechnology for miRNA are summarized. The types of signal amplification are given and the principles of amplification strategies are explained, including rolling circle amplification(RCA), catalytic hairpin assembly (CHA), hybridization chain reaction(HCR) and DNA walker. Subsequently, the application of these signal amplification methods in biosensing and bioimaging are covered and described. Finally, the challenges and the outlook of fluorescence signal amplification methods for miRNA detection are briefly commented.

Key words: Signal amplification, DNA nanotechnology, Fluorescence, miRNA detection, Biosensing and bioimaging