Determination of Amino Acids as Their N-Hydroxy succinimidyl-3-indolylacetate Derivatives by Reversed phase HPLC

Chemical Research in Chinese Universities ›› 1997, Vol. 13 ›› Issue (1): 14-20.

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Determination of Amino Acids as Their N-Hydroxy succinimidyl-3-indolylacetate Derivatives by Reversed phase HPLC

QIU Pei-hong¹, ZHANG Hua-shan², XU Guo-liang², CHENG Jie-ke²

1. Department of Chemistry, Wenzhou Medical College, Wenzhou 325003;
2. Department of chemistry, Wuhan University, Wuhan 430072

Received:1996-03-06 Online:1997-01-24 Published:2011-08-17
Supported by:
Supported by the National Natural Science Foundation of China.

Abstract

Abstract: The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N- hydroxy-succinimidyl-3-indolylacetate(SIIA). The derivatives of the amino acids were eluted by a mobile phase of methanol water containing 10 mmol/L H₃cit Na₂HPO₄ buffer of pH 3.5 and 1.2 mmol/L tetrabutylammonium bromide on an ODS column. The detection limits were pmol level or so, respectively, when a signal to noise ratio was 3. The method was applied to the determination of amino acids in tea sample with satisfactory results.

Key words: Liquid chromatography, N-Hydroxy succinimidyl-3-indolylacetate, Amino acid

QIU Pei-hong, ZHANG Hua-shan, XU Guo-liang, CHENG Jie-ke. Determination of Amino Acids as Their N-Hydroxy succinimidyl-3-indolylacetate Derivatives by Reversed phase HPLC[J]. Chemical Research in Chinese Universities, 1997, 13(1): 14-20.

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Determination of Amino Acids as Their N-Hydroxy succinimidyl-3-indolylacetate Derivatives by Reversed phase HPLC

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