Antibody Phage Library Screening Efficiency Measured by KD Values

Chemical Research in Chinese Universities ›› 2005, Vol. 21 ›› Issue (6): 677-679.

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Antibody Phage Library Screening Efficiency Measured by KD Values

WANG Hui-tang^1,2, SHAN Ya-ming¹, TANG Li-li¹, GAO Li-zeng¹, WANG Li-ping¹, LI Wei¹, LI Yu-xin²

1. College of Life Science, Jilin University, Changchun 130023, P. R. China;
2. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130021, P. R. China

Received:2004-11-08 Online:2005-11-23 Published:2011-08-06
Supported by:
Supported by the National Natural Science Foundation of China(No.29774010)

Abstract

Abstract: An antibody phage library was screened with two target molecules, IFNα-2a and FGFR-GST, and the KD value of each round of panning was measured.It was found that the apparent KD values decreased along with each additional panning round, which indicates the increase of the binding affinity between the phage and the target molecules.This result shows that the KD value is a reliable intrinsic parameter and a new method for screening efficiency detection is thus provided.

Key words: KD value, Antibody phage library, Screening efficiency, Target

WANG Hui-tang, SHAN Ya-ming, TANG Li-li, GAO Li-zeng, WANG Li-ping, LI Wei, LI Yu-xin. Antibody Phage Library Screening Efficiency Measured by KD Values[J]. Chemical Research in Chinese Universities, 2005, 21(6): 677-679.

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Antibody Phage Library Screening Efficiency Measured by KD Values

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