Fabrication of a Magnetite Nanoparticle-loaded Polymeric Nanoplatform for Magnetically Guided Drug Delivery

doi:10.1007/s40242-013-2134-7

Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (1): 103-109.doi: 10.1007/s40242-013-2134-7

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Fabrication of a Magnetite Nanoparticle-loaded Polymeric Nanoplatform for Magnetically Guided Drug Delivery

DING Guo-bin¹, LIU Hui-ying³, WANG Yan¹, Lü Yan-yun¹, WU Yi², GUO Yi¹, XU Li¹

1. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, College of Life Science, Jilin University, Changchun 130012, P. R. China;
2. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
3. School of Stomatology, Dalian Medical University, Dalian 116044, P. R. China

Received:2012-04-05 Revised:2012-05-10 Online:2013-02-01 Published:2013-01-23
Supported by:
Supported by the National Natural Science Foundation of China(Nos.30970719, 81000669), the Social Development Project of the Science and Technology Department of Jilin Province, China(No.20106031), the Project of Science and Technology Department of Changchun City, China(No.2009080-09SF02), the Specialized Research Fund for the Doctoral Program of Higher Education of China(No.20100061120077) and the China Postdoctoral Science Foundation(No.20100481048).

Abstract

Abstract:

We developed a magnetite nanoparticle-loaded polymeric nanoplatform for magnetically guided 10-hydroxycamptothecin(HCPT) delivery. The nanoplatform was fabricated by simultaneously incorporating magnetite nanoparticles(NPs) and HCPT into the polymer micelle self-assembled from methoxy polyethylene glycol- poly(D,L-lactide-co-glycolide)(MPEG-PLGA) copolymer. Successful loading of HCPT into the nanoplatform was confirmed by Fourier transform infrared(FTIR) spectroscopy. Subsequently, we examined the in vitro antitumor efficacy of free HCPT and nanoplatform against three different cancer cell lines—HeLa, A549 and HepG2. Flow cytometric analysis was conducted to reveal the cell apoptosis caused by free HCPT and nanoplatform. Finally, the magnetic targeting property of the nanoplatform was evaluated by a self-designed in vitro experiment.

Key words: Magnetite nanoparticle, 10-Hydroxycamptothecin(HCPT), Nanoplatform, Magnetic targeting

DING Guo-bin, LIU Hui-ying, WANG Yan, Lü Yan-yun, WU Yi, GUO Yi, XU Li. Fabrication of a Magnetite Nanoparticle-loaded Polymeric Nanoplatform for Magnetically Guided Drug Delivery[J]. Chemical Research in Chinese Universities, 2013, 29(1): 103-109.

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Fabrication of a Magnetite Nanoparticle-loaded Polymeric Nanoplatform for Magnetically Guided Drug Delivery

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