Actional Mechanism of Trifluoroacetic Acid for the Separation of Biopolymers by Reversed-phase Liquid Chromatography

Chemical Research in Chinese Universities ›› 1992, Vol. 8 ›› Issue (3): 207-211.

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Actional Mechanism of Trifluoroacetic Acid for the Separation of Biopolymers by Reversed-phase Liquid Chromatography

SHI Ya-li, GENG Xin-du

Department of Chemistry, Northwest University, Xi'an, 710069

Received:1991-04-18 Online:1992-08-24 Published:2011-09-09
Supported by:
Supported by the National Natrual Science Foundation of China

Abstract

Abstract: The present paper covers the actional mechanism of trifluoroacetic acid for the separation of biopolymers investigated by using the parameters of stoichiometric displacement model for retention(SDM-R) in reversed-phase liquid chromatography. It was found that the trifluoroacetic acid(TFA) may participate in, or stimulate the association among displacing agent molecules in mobile phase, and decrease the affinity of both the associate molecules of the displacing agent and the TFA-protein ion-pairing. The former dominates over the separation selectivity of biopolymers as the concentration of TFAis lower than a given value, and the two contrary functions partly offset to each other and the latter dominates as its concentration is greater than the given value.

Key words: Trifluoroacetic acid, Actional mechanism, Reversed-phase liquid chroma-tography, Separation, Biopolymers

SHI Ya-li, GENG Xin-du . Actional Mechanism of Trifluoroacetic Acid for the Separation of Biopolymers by Reversed-phase Liquid Chromatography[J]. Chemical Research in Chinese Universities, 1992, 8(3): 207-211.

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Actional Mechanism of Trifluoroacetic Acid for the Separation of Biopolymers by Reversed-phase Liquid Chromatography

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