Single Particle Colorimetric Acid Phosphatase Activity Assay with CeO2-modified Gold Nanoparticles

doi:10.1007/s40242-024-4024-6

Chemical Research in Chinese Universities ›› 2024, Vol. 40 ›› Issue (2): 320-325.doi: 10.1007/s40242-024-4024-6

• Articles • Previous Articles Next Articles

Single Particle Colorimetric Acid Phosphatase Activity Assay with CeO₂-modified Gold Nanoparticles

ZHU Tang Jiayao¹, YUAN Xiang¹, MAO Guojiang², WEI Lin¹, XIAO Lehui³

1. Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, National and Local Joint Engineering Laboratory for New Petrochemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China;
2. Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, P. R. China;
3. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China

Received:2024-01-29 Revised:2024-02-23 Online:2024-04-01 Published:2024-03-27
Contact: WEI Lin lwei@hunnu.edu.cn
Supported by:
This work was supported by the Natural Science Foundation of Hunan Province, China (No. 2022JJ40266) and the Open Research Fund of School of Chemistry and Chemical Engineering, Henan Normal University, China (No. 2022A04).

Abstract

Abstract: Acid phosphatase (ACP) is a ubiquitous phosphatase in living organisms. The abnormal variation of ACP is related to various diseases. Herein, we propose a colorimetric method based on CeO₂-modified gold core shell nanoparticles (Au@CeO₂ NPs) to analyze ACP activity with high sensitivity and specificity. In this design, 2-phospho-L-ascorbic acid trisodium salt (AAP) is dephosphorylated by ACP and produces reductive ascorbic acid (AA), which makes the CeO₂ shell decomposition. A remarkable blue shift of localized surface plasmon resonance peak (LSPR, from yellow to green) along with the scattering intensity ratio changes from individual Au@CeO₂ NPs are observed. ACP activity can be quantified by calculating the ratio changes of individual Au@CeO₂ NPs. This assay reveals limit of detection (LOD) of 0.044 mU/mL and the linear range of 0.05—5.0 mU/mL, which are much lower than most of spectroscopic measurements in bulk solution. Furthermore, the recovery measurements in real samples are satisfactory and the capacity for practical application is demonstrated. As a consequence, Au@CeO₂ NPs used in this assay will find new applications for the ultrasensitive detection of enzyme activity.

Key words: Single particle imaging, Dark field, Gold nanoparticle, Enzyme activity, Colorimetric detection

ZHU Tang Jiayao, YUAN Xiang, MAO Guojiang, WEI Lin, XIAO Lehui. Single Particle Colorimetric Acid Phosphatase Activity Assay with CeO₂-modified Gold Nanoparticles[J]. Chemical Research in Chinese Universities, 2024, 40(2): 320-325.

References

[1] Qin Y., Yang Y. H., He R., Zhou L. C., Zhang L., Chem. Res. Chinese Universities, 2022, 38 (6), 1497
[2] Zhang G. Q., Johnston T., Quin M. B., Schmidt-Dannert C., ACS Synth. Biol., 2019, 8, 1876
[3] Suea-Ngam A., Bezinge L., Mateescu B., Howes P. D., deMello J. A., Richards D. A., ACS Sens., 2020, 5, 2701
[4] Schlichtmann B. W., Kondru N., Hepker M. M., Kanthasamy A. G., Anantharam V., John M., Ban B., Mallapragada S. K., Narasimhan B., ACS Chem. Neurosci., 2020, 11, 4179
[5] Li Q. N., Qiang W. Z., Yuan J., Xiao L. H., Anal. Chem., 2023, 95, 7796
[6] Guo Y. Y., Li X. Q., Dong Y. M., Wang G. L., ACS Sustainable Chem. Eng., 2019, 7, 7572
[7] Han Y. X., Quan K. J., Chen J., Qiu H. D., Biosens. Bioelectron., 2020, 170, 112671
[8] Zhang G. Q., Chen Q. J., Sun J., Wang H. X., Han C. H., J. Basic Microbiol., 2013, 53, 868
[9] Yan X., Xia C., Chen B., Li Y. F., Gao P. F., Huang C. Z., Anal. Chem., 2020, 92, 2130
[10] Wang J., Lu Q. Y., Weng C. Y., Li X. Y., Yan X. Q., Yang W., Li B. Z., Zhou X. M., ACS Biomater. Sci. Eng., 2020, 6, 3132
[11] Hassan S. S. M., Sayour H. E. M., Kamel A. H., Anal. Chim. Acta, 2009, 640, 75
[12] Ahmed S. R., Chen A. C., ACS Appl. Nano Mater., 2020, 3, 9462
[13] Yuan X., Zhang H. L., Yuan X., Mao G. J. Wei L., Microchem. J., 2023, 184, 108133
[14] Liu J. X., Wang Y. F., Ma W. Y., Zong S. Y., Li J. Y., Chem. Res. Chinese Universities, 2022, 38 (6), 1446
[15] Wang F. Y., Li Y. L., Han Y. M., Ye Z. J., Wei L., Luo H. B., Xiao L. H., Anal. Chem., 2019, 91, 6329
[16] Su C., Bai L. L., Zhang H. B., Chang K. S., Li G. B., Li S. L., Chem. Res. Chinese Universities, 2019, 35 (1), 163
[17] Ye Z. J., Weng R., Ma Y. H., Wang F. Y., Liu H., Wei L., Xiao L. H., Anal. Chem., 2018, 90, 13044
[18] Sztandera K., Gorzkiewicz M., Klajnert-Maculewicz B., Mol. Phamaceutics, 2019, 16, 1
[19] Zhang Y. Q., Luo Q., Ding K., Liu S. G., Shi X. B., Sens. Actuat. B: Chem., 2021, 335, 129708
[20] Huang M. N., Fan Y. P., Yuan X., Wei L., Sens. Actuat. B: Chem., 2022, 353, 131135
[21] Ouyang Y. Z., Chen Y. C., Shang J. H., Sun S. J., Wang X. B., Huan S. Y., Xiong B., Zhang X. B., Anal. Chem., 2023, 95, 5009
[22] Tan L. L., Wen Z. H., Geng Z. Y., Jin Y. R., Wu H., Wang P. P., Chem. Res. Chinese Universities, 2023, 39 (4), 642
[23] Ye Z. J., Wang X., Xiao L. H., Anal. Chem., 2019, 91, 15327
[24] Ye Z. J., Wei L., Xiao L. H., Chem. Sci., 2019, 10, 5793
[25] Zhang Z. Q., Wang H. B., Li Y. X., Xie M. G., Li C. G., Lu H. Y., Peng Y., Shi Z., Chem. Res. Chinese Universities, 2022, 38(3), 750
[26] Yuan X., Zhang H. L., Cao H. J., Mao G. J., Wei L., Microchim. Acta, 2022, 189, 480
[27] Yuan X., Cao H. J., Zhang H. L., Mao G. J., Wei L., Spectrochim. Acta A, 2023, 299, 122888
[28] Creyer M. N., Jin Z. C., Retout M., Yim W. J., Zhou J. J., Jokerst J. V., Langmuir, 2022, 38, 14200
[29] Cibake-Ndaya C., Javahiraly N., Roiban L., Epicier T., Boubiche N., Valette S., Saury A., Brioude A., ACS Appl. Nano Mater., 2023, 6, 899
[30] Li L. L., Xu J. H., Liang X., Wu X. T., Wang X., Song S. Y., Zhang H. J., Chem. Res. Chinese Universities, 2023, 39(6), 921
[31] Jin H., Xu D., Tian C., Yue Y. H., Hua W. M., Gao Z., Chem. Res. Chinese Universities, 2022, 38(6), 1547
[32] Jiang F., Wang S. S., Liu B., Liu J., Wang L., Xiao Y., Xu Y. B., Liu X. H., ACS Catal., 2020, 10, 11493
[33] Qi. F., Han Y. M., Ye Z. J., Liu H., Wei L., Xiao L. H., Anal. Chem., 2018, 90, 11146
[34] Chen. Y. Y., Wang Z. Z., Hao X. L., Li F. L., Zheng Y. J., Zhang J. Z., Lin X. H., Weng S. H., Sens. Actuat. B: Chem., 2019, 297, 126784
[35] Li R., Sun Y. N., Jin L. H., Qiao X. H., Li C., Shen Y. H., Anal. Methods, 2021, 13, 809
[36] Wang F. Y., Han Y. M., Wang S. M., Ye Z. J., Wei L., Xiao L. H., Anal. Chem., 2019, 91, 11856

Single Particle Colorimetric Acid Phosphatase Activity Assay with CeO₂-modified Gold Nanoparticles

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHI Rui, WEI Shuxian, CHENG Shiqi, ZENG Jinmin, WANG Yilin, SHU Xin. Colorimetric Detection of Glucose Using WO₃ Nanosheets as Peroxidase-mimetic Enzyme [J]. Chemical Research in Chinese Universities, 2022, 38(4): 985-990.
[2]	ZONG Chen, LIU Guangnan, XU Wenhao, CHEN Jie, TANG Yun. Block Copolymer Supported Gold Nanoparticles Assemblies with Exposed Gold Surface [J]. Chemical Research in Chinese Universities, 2022, 38(4): 1118-1122.
[3]	LAN Weifei, HU Ruifeng, HUANG Danrong, DONG Xu, SHEN Gangyi, CHANG Shan, DAI Dongsheng. Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection [J]. Chemical Research in Chinese Universities, 2022, 38(2): 529-534.
[4]	LONG Qipeng, YU Hanyang, LI Zhe. Reconfigurable Plasmonic Nanostructures Controlled by DNA Origami [J]. Chemical Research in Chinese Universities, 2020, 36(2): 296-300.
[5]	DONG Pengfei, LI Na, ZHAO Haiyan, CUI Min, ZHANG Cong, HAN Hongyan, REN Jujie. POMs as Active Center for Sensitively Electrochemical Detection of Bisphenol A and Acetaminophen [J]. Chemical Research in Chinese Universities, 2019, 35(4): 592-597.
[6]	ZHENG Jingyi, FENG Rongrong, HE Caimei and LI Xiaoxia. Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement [J]. Chemical Research in Chinese Universities, 2018, 34(6): 952-958.
[7]	WANG Shumin, YAN Xiaoxia, ZHU Yan, DENG Dongmei, HE Haibo, LUO Liqiang. Au Nanoparticles Loaded on Hollow TiO₂ Microspheres with (001) Exposed Facets: a Strategy for Promoting Photocatalytic Performance [J]. Chemical Research in Chinese Universities, 2018, 34(5): 705-710.
[8]	FENG Huiyun, GAO Lei, YE Xinhui, WANG Lei, XUE Zechun, KONG Jinming, LI Lianzhi. Synthesis of a Heptapeptide and Its Application in the Detection of Mercury(II) Ion [J]. Chemical Research in Chinese Universities, 2017, 33(2): 155-159.
[9]	ZHANG Ya, QIAN Linlin, YIN Wei, HE Bin, LIU Fangmei, HOU Changjun, HUO Danqun, FA Huanbao. A Dual Read-out Molecularly Imprinted Composite Membrane Sensor Based on Zinc Porphyrin for the Detection of Dimethyl Methylphosphonate [J]. Chemical Research in Chinese Universities, 2016, 32(5): 725-730.
[10]	MA Xiaoyu, YANG Jianmao, CAI Wenshu, ZHU Guodong, LIU Jianyun. Preparation of Au Nanoparticles Decorated Polyaniline Nanotube and Its Catalytic Oxidation to Ascorbic Acid [J]. Chemical Research in Chinese Universities, 2016, 32(4): 702-708.
[11]	ZHOU Chao, MU Ying, YANG Meng-chao, WU Qing-qing, XU Wei, ZHANG Ying, JIN Wei, SONG Qi, WU Zhong-yu, JIN Qin-han. Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification [J]. Chemical Research in Chinese Universities, 2013, 29(3): 424-428.
[12]	HOU Qiong, WANG Jin-e, LIU Xia, LI Xiao-kun, MA Li-na, DUAN Wu-biao, WANG Zhen-xin. Detection of Lectins by Saccharide-gold Nanoparticle Conjugates [J]. Chemical Research in Chinese Universities, 2012, 28(6): 947-952.
[13]	TENG Hong, LIU Yang and YOU Tian-yan*. Facile Synthesis of Gold Nanoparticle-loaded Carbon Nanofiber Composites and Their Electrocatalytic Activity Towards Dopamine, Ascorbic Acid and Uric Acid [J]. Chemical Research in Chinese Universities, 2011, 27(3): 496-499.
[14]	WANG Jin-e, WANG Cheng-ke, LIU Dian-jun and WANG Zhen-xin*. Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with Living Cell [J]. Chemical Research in Chinese Universities, 2011, 27(2): 193-197.
[15]	LI Xiao-kun and WANG Zhen-xin*. Gold Nanoparticle-based Colorimetric Assay for Determination ofLead(II) in Aqueous Media [J]. Chemical Research in Chinese Universities, 2010, 26(2): 194-197.