Synthesis of a Heptapeptide and Its Application in the Detection of Mercury(II) Ion

doi:10.1007/s40242-017-6362-0

Chemical Research in Chinese Universities ›› 2017, Vol. 33 ›› Issue (2): 155-159.doi: 10.1007/s40242-017-6362-0

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Synthesis of a Heptapeptide and Its Application in the Detection of Mercury(II) Ion

FENG Huiyun¹, GAO Lei², YE Xinhui^1,4, WANG Lei¹, XUE Zechun¹, KONG Jinming³, LI Lianzhi¹

1. School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, P. R. China;
2. Zhong Yuan Academy of Biological Medicine, Liaocheng People's Hospital, Liaocheng 252000, P. R. China;
3. School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, P. R. China;
4. College of Innovation and Entrepreneurship, Liaocheng University, Liaocheng 252059, P. R. China

Received:2016-09-01 Revised:2017-01-11 Online:2017-04-01 Published:2017-02-08
Contact: LI Lianzhi,E-mail:lilianzhi1963@163.com E-mail:lilianzhi1963@163.com
Supported by:
Supported by the Scientific Research Foundation of Liaocheng University, China(No.318011513) and the National Undergraduate Training Program for Innovation and Entrepreneurship of Liaocheng University, China(No.201610447004).

Abstract

Abstract:

A new heptapeptide, Cys-Pro-Gly-Cys-Pro-Gly-Cys-CONH₂(P7), was prepared via Fmoc solid-phase peptide synthesis, purified by high performance liquid chromatography(HPLC) and characterized by mass spectrometry(MS). The interactions of P7 with metal ions were also investigated by spectroscopy and the results show that P7 has highly selective binding affinity to Hg(II). Based on this specific character, a simple, rapid and sensitive detection method for Hg(II) was developed. When the mixture solution of P7 and Hg(II) was added to colloidal gold nanoparticles(GNP), the color of GNP remained unchanged. On the contrary, the color of GNP changed from red to blue when the mixture solutions of P7 and other metal ions were added.

Key words: Peptide, Hg²⁺ detection, Gold nanoparticle, Absorption spectrum

FENG Huiyun, GAO Lei, YE Xinhui, WANG Lei, XUE Zechun, KONG Jinming, LI Lianzhi. Synthesis of a Heptapeptide and Its Application in the Detection of Mercury(II) Ion[J]. Chemical Research in Chinese Universities, 2017, 33(2): 155-159.

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Synthesis of a Heptapeptide and Its Application in the Detection of Mercury(II) Ion

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