Colorimetric Detection of Glucose Using WO3 Nanosheets as Peroxidase-mimetic Enzyme

doi:10.1007/s40242-021-1215-2

Abstract

Abstract: In the present work, WO₃ nanosheets(WO₃ NSs) were prepared by a facile method at room temperature. The obtained WO₃ NSs showed peroxidase-like activity, which could catalyze 3,3',5,5'-tetramethylbenzidine(TMB) to form a blue oxidation product(ox TMB) in the presence of H₂O₂. Based on this, convenient and sensitive colorimetric methods for the detection of H₂O₂ and glucose were established. The linear ranges for detecting H₂O₂ and glucose were 1-200 μmol/L and 1-100 μmol/L, respectively. The limits of the detection of H₂O₂ and glucose were as low as 0.79 and 0.96 μmol/L, respectively. This method was also successfully applied to the detection of glucose in urine samples. The detection result was consistent with that of the value detected by the clinical method, indicating the potential in clinical diagnosis and biomedical detection.

Key words: WO₃ nanosheet, Peroxidase-like activity, Colorimetric detection, H₂O₂, Glucose

SHI Rui, WEI Shuxian, CHENG Shiqi, ZENG Jinmin, WANG Yilin, SHU Xin. Colorimetric Detection of Glucose Using WO₃ Nanosheets as Peroxidase-mimetic Enzyme[J]. Chemical Research in Chinese Universities, 2022, 38(4): 985-990.

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Colorimetric Detection of Glucose Using WO₃ Nanosheets as Peroxidase-mimetic Enzyme

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