Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification

doi:10.1007/s40242-013-2371-9

Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (3): 424-428.doi: 10.1007/s40242-013-2371-9

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Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification

ZHOU Chao¹, MU Ying¹, YANG Meng-chao¹, WU Qing-qing², XU Wei³, ZHANG Ying¹, JIN Wei¹, SONG Qi¹, WU Zhong-yu¹, JIN Qin-han¹

1. Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, P. R. China;
2. Central Laboratory, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, P. R. China;
3. School of Mathematics and Science, Shijiazhuang University of Economics, Shijiazhuang 050031, P. R. China

Received:2012-09-24 Revised:2012-11-02 Online:2013-06-01 Published:2013-05-15
Contact: Ying Mu E-mail:muying@zju.edu.cn; qhjin@zju.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.31070772, 31270907), the Doctoral Program of Higher Education of China(No.20090101110136), the Science and Technology Programs of Zhejiang Province of China(No.2011C37029) and the Fund of State Key Laboratory of Industrial Control Technology of China.

Abstract

Abstract:

We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA. It is based on loop-mediated isothermal amplification(LAMP). Plain gold nanoparticles(AuNPs) are used to indicate the occurrence of LAMP. The amplified product is mixed with AuNPs in an optimized ratio, at which the deoxyribonucleotides(dNTPs) bind to the AuNPs via ligand-metal interactions and thus enhance AuNPs stability. If a target DNA is amplified, the dramatic reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for the identification of isothermal products, this method is simple and can be readily applied on site where instrumentation is inadequate or even lacking.

Key words: Label-free, Gold nanoparticle, Loop-mediated isothermal amplification, Naked eyes

ZHOU Chao, MU Ying, YANG Meng-chao, WU Qing-qing, XU Wei, ZHANG Ying, JIN Wei, SONG Qi, WU Zhong-yu, JIN Qin-han. Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification[J]. Chemical Research in Chinese Universities, 2013, 29(3): 424-428.

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Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification

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