NIR-II Excitation and NIR-I Emission Based Two-photon Fluorescence Lifetime Microscopic Imaging Using Aggregation-induced Emission Dots

doi:10.1007/s40242-021-0405-2

Chemical Research in Chinese Universities ›› 2021, Vol. 37 ›› Issue (1): 171-176.doi: 10.1007/s40242-021-0405-2

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NIR-II Excitation and NIR-I Emission Based Two-photon Fluorescence Lifetime Microscopic Imaging Using Aggregation-induced Emission Dots

LIU Wen^1,2, ZHANG Yuhuang¹, QI Ji³, QIAN Jun¹, TANG Ben Zhong³

1. State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou 310058, P. R. China;
2. Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology, Guangzhou 510640, P. R. China;
3. Department of Chemistry, The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study and Department of Chemical and Biological Engineering and Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P. R. China

Received:2020-12-01 Revised:2020-12-25 Online:2021-02-01 Published:2021-02-03
Contact: QIAN Jun E-mail:qianjun@zju.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos.61735016, 61975172), the Natural Science Foundation of Zhejiang Province of China(No.LR17F050001), the Fundamental Research Funds for the Central Universities of China(No.2020-KYY-511108-0007) and the Open Fund of Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregate, South China University of Technology, China(No.2019B030301003).

Abstract

Abstract: Near-infrared(NIR) lights are powerful tools to conduct deep-tissue imaging since NIR-I wavelengths hold less photon absorption and NIR-II wavelengths serve low photon scattering in the biological tissues compared with visible lights. Two-photon fluorescence lifetime microscopy(2PFLM) can utilize NIR-II excitation and NIR-I emission at the same time with the assistance of a well-designed fluorescent agent. Aggregation induced emission(AIE) dyes are famous for unique optical properties and could serve a large two-photon absorption(2PA) cross-section as aggregated dots. Herein, we report two-photon fluorescence lifetime microscopic imaging with NIR-II excitation and NIR-I emission using a novel deep-red AIE dye. The AIE-gens held a 2PA cross-section as large as 1.61×10⁴ GM at 1040 nm. Prepared AIE dots had a two-photon fluorescence peak at 790 nm and a stable lifetime of 2.2 ns under the excitation of 1040 nm femtosecond laser. The brain vessels of a living mouse were vividly reconstructed with the two-photon fluorescence lifetime information obtained by our home-made 2PFLM system. Abundant vessels as small as 3.17 μm were still observed with a nice signal-background ratio at the depth of 750 μm. Our work will inspire more insight into the improvement of the working wavelength of fluorescent agents and traditional 2PFLM.

Key words: Near-infrared, Brain imaging, Aggregation-induced emission, Two-photon fluorescence lifetime microscopic imaging

LIU Wen, ZHANG Yuhuang, QI Ji, QIAN Jun, TANG Ben Zhong. NIR-II Excitation and NIR-I Emission Based Two-photon Fluorescence Lifetime Microscopic Imaging Using Aggregation-induced Emission Dots[J]. Chemical Research in Chinese Universities, 2021, 37(1): 171-176.

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NIR-II Excitation and NIR-I Emission Based Two-photon Fluorescence Lifetime Microscopic Imaging Using Aggregation-induced Emission Dots

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