Cytotoxicity of NiO Nanoparticles and Its Conversion Inside Chlorella vulgaris

doi:10.1007/s40242-017-6246-3

Abstract

Abstract:

Cytotoxicities of nickel oxide nanoparticles(NiO NPs) with average diameter of 20 nm were investigated on cultured Chlorella vulgaris. Alga growth-inhibition tests were taken and ultrastructure changes of the microalgae were characterized with transmission electron microscopy(TEM). The biological interface conversion effect between NiO nanoparticles and Chlorella vulgaris was studied by X-ray diffraction(XRD), high-resolution transmission electron microscopy(HRTEM) and X-ray photoelectron spectroscopy(XPS). The results indicated that the NiO nanoparticles had severe inhibitory effect on the growth of microalgae, with a 96 h EC₅₀ value of 31.4 mg/L. Under the exposure to NiO NPs suspensions, Chlorella vulgaris cells showed plasmolysis with a shriveled cell shape, disrupted plasma membrane, leaked cytosol and disordered thylakoid grana lamella. The NiO NPs were aggregated and partially reduced to Ni⁰ inside the Chlorella vulgaris. The bioaccumulation and bio-reduction ability of Chlorella vulgaris provide us with a possible strategy of remediation of aquatic pollution conducted by toxic metal oxide nanoparticles.

Key words: Chlorella vulgaris, Nanoparticle, Cytotoxicity, Conversion

LI Yongqing, XIAO Ran, LIU Zonglai, LIANG Xiujuan, FENG Wei. Cytotoxicity of NiO Nanoparticles and Its Conversion Inside Chlorella vulgaris[J]. Chemical Research in Chinese Universities, 2017, 33(1): 107-111.

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