Preparation of Functionalized Fe3O4@SiO2 Magnetic Nanoparticles for Monoclonal Antibody Purification

doi:10.1007/s40242-016-6251-y

Chemical Research in Chinese Universities ›› 2016, Vol. 32 ›› Issue (6): 889-894.doi: 10.1007/s40242-016-6251-y

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Preparation of Functionalized Fe₃O₄@SiO₂ Magnetic Nanoparticles for Monoclonal Antibody Purification

HOU Xuemei¹, ZHAO Changjie¹, TIAN Yanlong², DOU Shuliang², ZHANG Xiang², ZHAO Jiupeng¹

1. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, P. R. China;
2. Center for Composite Materials, Harbin Institute of Technology, Harbin 150001, P. R. China

Received:2016-06-15 Revised:2016-09-22 Online:2016-12-01 Published:2016-10-24
Contact: ZHAO Jiupeng, E-mail:jiupengzhao@126.com E-mail:jiupengzhao@126.com
Supported by:
Supported by the National Natural Science Foundation of China(Nos.51572058, 91216123, 51174063), the Natural Science Foundation of Heilongjiang Province, China(No.E201436), the International Science & Technology Cooperation Program of China(Nos.2013DFR10630, 2015DFE52770) and the Specialized Research Fund for the Doctoral Program of Higher Education of China(No.SRFDP 20132302110031).

Abstract

Abstract:

Magnetic Fe₃O₄@SiO₂ nanoparticles with superparamagnetic properties were prepared via a reverse mi-croemulsion method at room temperature. The as-prepared samples were characterized by transmission electron mi-croscopy(TEM), X-ray diffractometry(XRD), and vibrating sample magnetometry(VSM). The Fe₃O₄@SiO₂ nanoparticles were modified by (3-aminopropyl)triethoxysilane(APTES) and subsequently activated by glutaraldehyde(Glu). Protein A was successfully immobilized covalently onto the Glu activated Fe₃O₄@SiO₂ nanoparticles. The adsorption capacity of the nanoparticles was determined on an ultraviolet spectrophotometer(UV) and approximately up to 203 mg/g of protein A could be uniformly immobilized onto the modified Fe₃O₄@SiO₂ magnetic beads. The core-shell of the Fe₃O₄@SiO₂ magnetic beads decorated with protein A showed a good binding capacity for the chime-ric anti-EGFR monoclonal antibody(anti-EGFR mAb). The purity of the anti-EGFR mAb was analyzed by virtue of HPLC. The protein A immobilized affinity beads provided a purity of about 95.4%.

Key words: Magnetic, Fe₃O₄@SiO₂, Protein A, Nanoparticle, Monoclonal antibody

HOU Xuemei, ZHAO Changjie, TIAN Yanlong, DOU Shuliang, ZHANG Xiang, ZHAO Jiupeng. Preparation of Functionalized Fe₃O₄@SiO₂ Magnetic Nanoparticles for Monoclonal Antibody Purification[J]. Chemical Research in Chinese Universities, 2016, 32(6): 889-894.

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Preparation of Functionalized Fe₃O₄@SiO₂ Magnetic Nanoparticles for Monoclonal Antibody Purification

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