Chemical Research in Chinese Universities ›› 2014, Vol. 30 ›› Issue (4): 566-571.doi: 10.1007/s40242-014-4035-9

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Differential Proteomics Analysis on Fluoride-resistant Streptococcus mutans by Label-free Quantitation

ZHAO Hongyan1, ZHANG Zhimin1, ZHU Laikuan2, LI Zhenling1, LI Penglian1, LIU Jiabai1, ZHANG Zhiying1   

  1. 1. School of Stomatology, Jilin University, Changchun 130021, P. R. China;
    2. Shanghai Key Laboratory of Stomatology, The Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, P. R. China
  • Online:2014-08-01 Published:2014-05-19
  • Contact: ZHANG Zhimin E-mail:zhangzm1964@sina.com
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.81170945, 81200774).

Abstract:

To figure out the changed biological characteristics of a fluoride-resistant strain compared with those of the parent sensitive strain of Streptococcus mutans(S. mutans) with the help of differential proteomics analysis, sixty- seven proteins with obvious differential expression were identified in fluoride-resistant strain. Among them are the important virulence factors causing caries, including glucosyltransferase-S, glucosyltransferase-I, glucosyltransferase-Si, major cell-surface adhesin Pac, F-ATPase β-subunit, Ffh, molecular chaperone DnaK, chaperonin GroEL, and the lactate dehydrogenase. We obtained the differential proteomics expression profiles of the fluoride-resistant strain(UA159-FR) and its parental strain(UA159) of S. mutans. Several important virulence factors bringing about caries were identified to have differential expressions, among which the expression of lactate dehydrogenase in fluoride-resistant strain was enhanced compared to that in its parent strain. The results could provide the basis for analysing the functions of proteins encoded by caries-related genes from the fluoride-resistant S. mutans strain.

Key words: Fluoride-resistant, Streptococcus mutans, Differential proteomics, Label-free quantitation