Rapid Absolute Determination Platform of Nucleic Acid for Point-of-care Testing

doi:10.1007/s40242-015-4503-x

Chemical Research in Chinese Universities ›› 2015, Vol. 31 ›› Issue (4): 519-525.doi: 10.1007/s40242-015-4503-x

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Rapid Absolute Determination Platform of Nucleic Acid for Point-of-care Testing

SONG Bofan, JIN Wei, SONG Qi, JIN Qinhan, MU Ying

Research Center for Analytical Instrumentation, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, P. R. China

Received:2014-12-24 Revised:2015-03-23 Online:2015-08-01 Published:2015-04-20
Contact: JIN Wei, MU Ying E-mail:jinweimy@zju.edu.cn;muying@zju.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China (No.31270907), the National Key Foundation for Exploring Scientific Instrument of China(No.2013YQ470781) and the Scientific Research Project of Shanghai City Science and Technology Committee, China(No.14495810400).

Abstract

Abstract:

Nowadays nucleic acid tests are promising to be considered as point-of-care testing(POCT). However, no such devices are currently available that can perform all the functions, including absolute nucleic acid determination, worldwide on-site detection, rapid analysis and real-time results reporting via ubiquitous mobile networks simultaneously with full package and automated means of measurement. In this study, we presented a compact low-cost portable POC automated testing platform with all attributes mentioned above. A disposable self-priming compartmentalization(SPC) microfluidic chip is used to conduct isothermal amplification. The platform also includes a micro-computer controlled heating unit, an inexpensive optical imaging setup, and a mobile device with customized software. It may become a useful tool for the rapid on-site detection of infectious diseases as well as other pathogens.

Key words: Point-of-care testing, Microfluidic chip, Loop-mediated isothermal amplification, Mobile medicine

SONG Bofan, JIN Wei, SONG Qi, JIN Qinhan, MU Ying. Rapid Absolute Determination Platform of Nucleic Acid for Point-of-care Testing[J]. Chemical Research in Chinese Universities, 2015, 31(4): 519-525.

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Rapid Absolute Determination Platform of Nucleic Acid for Point-of-care Testing

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