Immobilization of Hemoglobin on Cobalt Nanoparticles-modified Indium Tin Oxide Electrode: Direct Electrochemistry and Electrocatalytic Activity

doi:10.1007/s40242-013-2273-x

Abstract

Abstract:

A cobalt nanoparticles-attached indium tin oxide(CoNPs/ITO) electrode was applied to the immobilization of hemoglobin(Hb) and an Hb modified CoNPs/ITO electrode(Hb/CoNPs/ITO) was prepared. The direct electron transfer of Hb was shown by the well-behaved voltammetric responses for Hb/CoNPs/ITO electrode and the effects of scan rate and pH value were observed. Based on the catalytic activity of Hb immobilized on the CoNPs/ITO electrode toward the reduction of H₂O₂, a mediator-free H₂O₂ sensor was developed. A linear relationship existed between the catalytic current and the H₂O₂ concentration in a range of 1.0―100.0 μmol/L with a detection limit (S/N=3) of 0.2 μmol/L.

Key words: Cobalt nanoparticle, Indium tin oxide electrode, Direct electron transfer, Hemoglobin, Electrocatalysis, Hydrogen peroxide

LI Shuo-qi, HU Jing-bo. Immobilization of Hemoglobin on Cobalt Nanoparticles-modified Indium Tin Oxide Electrode: Direct Electrochemistry and Electrocatalytic Activity[J]. Chemical Research in Chinese Universities, 2013, 29(3): 563-567.

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