High-efficient Isolation of Plant Viral RNA via TMAOH-modified Fe3O4 Magnetic Nanoparticles

doi:10.1007/s40242-014-3269-x

Chemical Research in Chinese Universities ›› 2014, Vol. 30 ›› Issue (1): 23-26.doi: 10.1007/s40242-014-3269-x

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High-efficient Isolation of Plant Viral RNA via TMAOH-modified Fe₃O₄ Magnetic Nanoparticles

TANG Yan^1,3, ZHAO Xiaoli², CHU Linya^1,4, SUN Ning^1,2, LIU Renxiao¹, DENG Congliang², GE Guanglu¹

1. Key Laboratory of Measurement and Standardization for Nanotechnology of Chinese Academy of Sciences, National Center for Nanoscience and Technology, Beijing 100190, P. R. China;
2. Beijing Entry-exit Inspection and Quarantine Bureau, Beijing 100026, P. R. China;
3. University of Chinese Academy of Sciences, Beijing 100049, P. R. China;
4. School of Materials Science and Engineering, Shandong University, Jinan 250100, P. R. China

Received:2013-06-26 Revised:2013-11-06 Online:2014-02-01 Published:2013-11-25
Contact: DENG Congliang,E-mail:gegl@nanoctr.cn; GE Guanglu,E-mail:dengcl@bjciq.gov.cn E-mail:gegl@nanoctr.cn; dengcl@bjciq.gov.cn
Supported by:
Supported by the National Basic Research and Development Program of China(No.2011CB932803) and the Special Fund of the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China(No.201110035).

Abstract

Abstract:

Magnetic nanoparticles show great potential in RNA enrichment and separation for rapid detection of viral infection. Fundamental studies on the interaction between RNA and nanoparticles with uniform size and surface property are necessary for designing better adsorbent and optimizing the conditions. In this study, monodispersed superparamagnetic magnetite(Fe₃O₄) nanoparticles were synthesized by thermal decomposition and modified with tetramethylammonium hydroxide[N(CH₃)₄OH, TMAOH] that become highly dispersible and stable in water. High-efficiency plant viral RNA adsorption onto TMAOH/Fe₃O₄ nanoparticles in the extracted solution of plant leaves was demonstrated. The changes of surface charge of TMAOH on the Fe₃O₄ nanoparticles with pH contribute to the RNA adsorption and elution. Separating viral RNA with magnetic nanoparticles could be a simple, quick and highly efficient method.

Key words: Magnetic nanoparticle, Fe₃O₄, Tetramethylammonium hydroxide(TMAOH), RNA adsorption

TANG Yan, ZHAO Xiaoli, CHU Linya, SUN Ning, LIU Renxiao, DENG Congliang, GE Guanglu. High-efficient Isolation of Plant Viral RNA via TMAOH-modified Fe₃O₄ Magnetic Nanoparticles[J]. Chemical Research in Chinese Universities, 2014, 30(1): 23-26.

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High-efficient Isolation of Plant Viral RNA via TMAOH-modified Fe₃O₄ Magnetic Nanoparticles

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