Dose-dependent Cytotoxicity and Oxidative Stress Induced by “Naked” Fe3O4 Nanoparticles in Human Hepatocyte

Abstract

Abstract: The dose-dependent cytotoxicity effect on human hepatocyte(HL-7702 cells) induced by “naked” Fe3O4 nanoparticles was assessed through cell viabilities and lactate dehydrogenase(LDH) activities. Three important oxidative indexes of the cells by glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) and malondialdehyde( MDA) were determined. The good correlation of the cell viabilities with their GSH-Px, SOD and MDA levels indicated that the cytotoxicity is related to activation of oxidative stress induced by Fe₃O₄ nanoparticles. The oxidative stress also leads to corresponding DNA damage in a similar dose-dependent manner, followed by the changes of cell cycle and cell apoptosis. Such work provides important experimental data for the safety evaluation of superparamagnetic Fe₃O₄ nanoparticles.

Key words: Superparamagnetic, Fe₃O₄, Cytotoxicity, Oxidative stress, DNA damage, Cell cycle, Cell apoptosis

LIN Xin-li, ZHAO Shu-hua, ZHANG Long, HU Gui-qin, SUN Zhi-wei, YANG Wen-sheng. Dose-dependent Cytotoxicity and Oxidative Stress Induced by “Naked” Fe₃O₄ Nanoparticles in Human Hepatocyte[J]. Chemical Research in Chinese Universities, 2012, 28(1): 114-118.

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Dose-dependent Cytotoxicity and Oxidative Stress Induced by “Naked” Fe₃O₄ Nanoparticles in Human Hepatocyte

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