Electrospun Poly(acrylonitrile-co-acrylic acid) Nanofibrous Membranes for Catalase Immobilization: Effect of Porphyrin Filling on the Enzyme Activity

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (2): 339-344.

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Electrospun Poly(acrylonitrile-co-acrylic acid) Nanofibrous Membranes for Catalase Immobilization: Effect of Porphyrin Filling on the Enzyme Activity

KE Bei-bei, WAN Ling-shu, HUANG Xiao-jun and XU Zhi-kang*

Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China

Received:2010-06-28 Revised:2010-08-16 Online:2011-03-25 Published:2011-03-09
Contact: XU Zhi-kang E-mail:xuzk@zju.edu.cn
Supported by:
Supported by the National High-Tech Research and Development Program of China(No.2007AA10Z301) and the National Natural Science Foundation of China for Distinguished Young Scholars(No.50625309).

Abstract

Abstract: Porphyrin-filled nanofibrous membranes were facilely prepared by electrospinning of the mixtures of poly(acrylonitrile-co-acrylic acid)(PANCAA) and porphyrins. 5,10,15,20-Tetraphenylporphyrin(TPP) and its metalloderivatives(ZnTPP and CuTPP) were studied as filling mediators for the immobilization of redox enzyme. Results indicate that the introduction of TPP, ZnTPP and CuTPP improves the retention activity of the immobilized catalase. Among these three porphyrins, the ZnTPP-filled PANCAA nanofibrous membrane exhibits an activity retention of 93%, which is an exciting improvement. This improvement is attributed to both the strong catalase-porphyrin affinity and the possible facilitated electron transfer induced by the porphyrin as evidenced by quartz crystal microbalance (QCM) and fluorescence spectroscopy studies.

Key words: Enzyme immobilization, Nanofibrous membrane, Electrospinning, Quartz crystal microbalance(QCM), Porphyrin

KE Bei-bei, WAN Ling-shu, HUANG Xiao-jun and XU Zhi-kang*. Electrospun Poly(acrylonitrile-co-acrylic acid) Nanofibrous Membranes for Catalase Immobilization: Effect of Porphyrin Filling on the Enzyme Activity[J]. Chemical Research in Chinese Universities, 2011, 27(2): 339-344.

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Electrospun Poly(acrylonitrile-co-acrylic acid) Nanofibrous Membranes for Catalase Immobilization: Effect of Porphyrin Filling on the Enzyme Activity

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