Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers

doi:10.1007/s40242-021-1104-8

Chemical Research in Chinese Universities ›› 2021, Vol. 37 ›› Issue (3): 522-527.doi: 10.1007/s40242-021-1104-8

• Articles • Previous Articles Next Articles

Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers

FENG Zhendong^1,2, ZHANG Xiaopei^1,2, LIU Na^1,2, WANG Yue^1,2, ZHOU Ziyi^1,2, GLEBOV O. Oleg^1,3, WU Tong^1,2

1. Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, Qingdao 266071, P. R. China;
2. Qingdao Medical College, Qingdao University, Qingdao 266071, P. R. China;
3. Department of Old Age Psychiatry, The Institute of Psychiatry, Psychology & Neuroscience, King's College London, De Crespigny Park, Denmark Hill, London SE5 8AF, UK

Received:2021-03-14 Revised:2021-04-28 Online:2021-06-01 Published:2021-05-31
Contact: WU Tong E-mail:twu@qdu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China(No.82001970) and the Startup Funds from Qingdao University, China (No.DC2000002357).

Abstract

Abstract: We report a general strategy to develop injectable welded nanofibers to facilitate the outgrowth and extension of neurites. In this case, nonwoven mats of uniaxially aligned poly(caprolactone)(PCL) nanofibers were firstly cut into several small pieces with fixed fiber lengths of 25, 50 and 100 μm, respectively, using a cryotome. A tissuelyser was employed to homogenize and disperse the short nanofibers to a homogeneous suspension. By tuning treatment duration from 100 s to 400 s, the temperature of the suspension was brought close to the melting point of PCL. As such, the short nanofibers were welded at their cross points while the fibers far away from the cross points remain the original structures. We showed that the viability of neuroblastoma SH-SY5Y cells and their neurite outgrowth and extension were enhanced with the use of such welded short nanofibers. Taken together, this study provides a simple way to generate injectable welded nanofibers, holding potential in affecting neurite outgrowth and extension for nerve repair, in particular, in the central nervous system.

Key words: Electrospinning, Nanofiber, Welding, Injectable, Neurite outgrowth

FENG Zhendong, ZHANG Xiaopei, LIU Na, WANG Yue, ZHOU Ziyi, GLEBOV O. Oleg, WU Tong. Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers[J]. Chemical Research in Chinese Universities, 2021, 37(3): 522-527.

References 19

[1]	Koprivica V., Cho K., Park J., Yiu G., Atwal J., Gore B., Kim J., Lin E., Tessier-Lavigne M., Chen D., He Z., Science, 2005, 310(5745), 106
[2]	Tran A., Warren P., Silver J., Physiol. Rev., 2018, 98(2), 881
[3]	Schwab J., Failli V., Chedotal A., The Lancet, 2005, 365(9476), 2055
[4]	Mukhopadhyay G., Doherty P., Walsh F., Crocker P., Filbin M., Neuron, 1994, 13(3), 757
[5]	Sridhar R., Lakshminarayanan R., Madhaiyan K., Barathi V. A., Lim K. H. C., Ramakrishna S., Chem. Sov. Rev., 2015, 44(3), 790
[6]	Xue J., Niu Y., Gong M., Shi R., Chen D., Zhang L., Lvov Y., ACS Nano, 2015, 9(2), 1600
[7]	Zhang H., Jia X., Han F., Zhao J., Zhao Y., Fan Y., Yuan X., Biomaterials, 2013, 34(9), 2202
[8]	Fujihara K., Kotaki M., Ramakrishna S., Biomaterials, 2005, 26(19), 4139
[9]	Kumbar S., Nukavarapu S., James R., Nair L., Laurencin C., Biomaterials, 2008, 29(30), 4100
[10]	Pektok E., Nottelet B., Tille J., Gurny R., Kalangos A., Moeller M., Walpoth B., Circulation, 2008, 118(24), 2563
[11]	Koh H., Yong T., Chan C., Ramakrishna S., Biomaterials, 2008, 29(26), 3574
[12]	Xie J., Liu W., MacEwan M., Bridgman P., Xia Y., ACS Nano, 2014, 8(2), 1878
[13]	Schnell E., Klinkhammer K., Balzer S., Brook G., Klee D., Dalton P., Mey J., Biomaterials, 2007, 28(19), 3012
[14]	Niu Y., Chen K., He T., Yu W., Huang S., Xu K., Biomaterials, 2014, 35(14), 4266
[15]	Wu T., Xue J., Li H., Zhu C., Mo X., Xia Y., ACS Appl. Mater Interfaces, 2018, 10(10), 8536
[16]	Wu T., Li H., Xue J., Mo X., Xia Y., Angew. Chem. Int. Ed., 2019, 58, 16416
[17]	Elnaggar M., El-Fawal H., Allam N., Mater. Sci. Eng. C, 2021, 119, 111550
[18]	McMurtrey R. J., J. Neural. Eng., 2014, 11(6), 066009
[19]	Wu T., Xue J., Xia Y., Angew. Chem. Int. Ed., 2020, 59, 15626

Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers

Knowledge

Abstract

Cite this article

share this article

References 19

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Bolun, CHAO Danming, WANG Ce. Piezoelectric Nanogenerator Based on Electrospun Cellulose Acetate/Nanocellulose Crystal Composite Membranes for Energy Harvesting Application [J]. Chemical Research in Chinese Universities, 2022, 38(4): 1005-1011.
[2]	YANG Siran, AI Feixue, LI Ziping, ZHAO Guiyan, BI Yanfeng. N-Doped Carbon Nanofibers Encapsulating CoO@Co₉S₈ Nanoparticles: Preparation from S-Rich Co₃₂ Coordination Cluster Precursors by Electrospinning and Application for Superior Li-ion Storage [J]. Chemical Research in Chinese Universities, 2022, 38(2): 603-608.
[3]	WANG Jun, FU Wanlin, XU Wanlin, WU Min, SUN Yueming, DAI Yunqian. Oxide Nanofibers as Catalysts Toward Energy Conversion and Environmental Protection [J]. Chemical Research in Chinese Universities, 2021, 37(3): 366-378.
[4]	HUANG Hedong, GUO Zeyu, YANG Pengyan, CHEN Peng, Wu Jie. Electrical Conductivity, Oil Absorption and Electric Heating of Carbon Black-modified Carbon Nanofibers [J]. Chemical Research in Chinese Universities, 2021, 37(3): 541-548.
[5]	BAI Shan, ZHANG Xiangyu, ZANG Leilei, YANG Songze, CHEN Xiaoqi, YUAN Xiaoyan. Electrospinning of Biomaterials for Vascular Regeneration [J]. Chemical Research in Chinese Universities, 2021, 37(3): 394-403.
[6]	QIN Mei, LIU Daqing, DAI Zhang, MENG Xin, LIU Guosai, LIU Hao, HUANG Xiaowei, YAN Xu, CHEN Shaojuan. One Step Fabrication and Application of Antibacterial Electrospun Zein/Cinnamon Oil Membrane Wound Dressing via In situ Electrospinning Process [J]. Chemical Research in Chinese Universities, 2021, 37(3): 464-469.
[7]	XIANG Huilin, ZHONG Lingling, REN Yongqing, LIU Dongmei, ZHU Zhigao, XU Ying, WANG Yu, WANG Wei. Superhydrophobized Polyacrylonitrile/Hierarchicall-FeOOH Nanofibrous Membrane for High-salinity Water Treatment in Membrane Distillation [J]. Chemical Research in Chinese Universities, 2021, 37(3): 470-479.
[8]	CAO Wenhao, WANG Caifeng, WANG Shuai, ZHANG Yang, ZHAO Ruisheng. Preparation of Photoresponsive PAN-NH₂@EPESP Fiber Films with Mechanical Stability for Regulating Wettability and Micro-environment Humidity [J]. Chemical Research in Chinese Universities, 2021, 37(3): 512-521.
[9]	Winges FATIMA, Muhammad TARIQUE, LI Min, CHEN Mingyi, Muzamil KHATRI, Muhammad Nauman SARWAR, Icksoo KIM, Farooq AHMED, Zeeshan KHATRI, CHEN Rouxi, WEI Kai. Reactive Dyeing of Electrospun Cellulose Nanofibers by Pad-steam Method [J]. Chemical Research in Chinese Universities, 2021, 37(3): 535-540.
[10]	LUO Yu, GONG Xiaoyan Shawn, XU Zhiwei, MENG Kai, ZHANG Ke-Qin, ZHAO Huijing. PTFE Electrospun Stent Graft——Preparation, Properties and Its Industrialization Prospect [J]. Chemical Research in Chinese Universities, 2021, 37(3): 589-597.
[11]	HOU Lanlan, LIU Jingchong, LI Dianming, GAO Yuan, WANG Yaqiong, HU Rongjun, REN Wen, XIE Shuixiang, CUI Zhimin, WANG Nü. Electrospinning Janus Nanofibrous Membrane for Unidirectional Liquid Penetration and Its Applications [J]. Chemical Research in Chinese Universities, 2021, 37(3): 337-354.
[12]	MA Yuying, HE Dayong, LIU Jiadi, WANG Yuannan, YANG Mei, WANG Hao, QIU Ju, LI Wenyan, LI Yongxin, WANG Ce. Adsorption and Visible Light Photocatalytic Degradation of Electrospun PAN@W₁₈O₄₉ Nanofibers [J]. Chemical Research in Chinese Universities, 2021, 37(3): 428-435.
[13]	LI Wenyan, LI Yanzi, LIU Jiadi, CHAO Shen, YANG Tianyi, LI Lijuan, WANG Ce, LI Xiang. A Novel Hollow Carbon@MnO₂ Electrospun Nanofiber Adsorbent for Efficient Removal of Pb²⁺ in Wastewater [J]. Chemical Research in Chinese Universities, 2021, 37(3): 496-504.
[14]	JIAN Shaoju, TIAN Zhiwei, ZHANG Kaiyin, DUAN Gaigai, YANG Weisen, JIANG Shaohua. Hydrothermal Synthesis of Ce-doped ZnO Heterojunction Supported on Carbon Nanofibers with High Visible Light Photocatalytic Activity [J]. Chemical Research in Chinese Universities, 2021, 37(3): 565-570.
[15]	WANG Ruifang, YU Xi, LI Zhenyu, CHEN Jingyu, JIANG Tingting. Partial P-Type Metal Ions Doping Induced Variation of Both Crystal Structure and Oxygen Vacancy Within Cu/SnO₂ Metastable Solid Solution Nanofibers for Highly Sensitive C₂H₂ Sensor [J]. Chemical Research in Chinese Universities, 2021, 37(3): 584-588.