CRCU

Chemical Research in Chinese Universities ›› 2005, Vol. 21 ›› Issue (1): 24-27.

• Articles • Previous Articles     Next Articles

Electrochemical Study on the Interaction Betwwen Neutral Red and DNA

NIU Shu-yan1,2, ZHANG Shu-sheng2, SHI Xin2, JIAO Kui2   

  1. 1. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, P. R. China;
    2. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China
  • Received:2003-11-25 Online:2005-01-24 Published:2011-07-27
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20275020, 20475030), the Natural Science Foundation of Shandong Province(No.Z2002B02) and the National Key Laboratory Foundation of the Electroanalytical Chemistry of Changchun Institute of Applied Chemistry(SKLEAC 2004-8).

PDF (PC)

Cited

18

Abstract: A voltammetric study of the interaction of neutral Red(NR) with DNA at a gold electrode in a phosphate buffer solution is described.After adding DNA in an NR solution, the reduction peak current of NR decreases.The binding mechahisms of NR to DNA in different pH ranges are different.The reduction peak potential of NR in a pH 7.0 phosphate buffer solution in the presence of DNA shifts positively, indicating that the binding of NR to DNA is intercalation action, but at pH=6.0 the reduction peak potential of NR shifts negatively, indicating that the binding of NR to DNA is electrostatic action.The formed complexes are DNA-NR when [NR]/[DNA]<0.18 and DNA-3NR when [NR]/[DNA]>0.35, respectively.

Key words: Neutral Red, DNA, Cyclic voltammetry, Intercalation