Studies on Determining Method of Trehalose

Chemical Research in Chinese Universities ›› 2000, Vol. 16 ›› Issue (1): 10-14.

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Studies on Determining Method of Trehalose

LIU Tie-min¹, LIU Ying²

1. State Key Laboratory of Enzyme Engineering, Jilin University, Changchun 130023, P. R. China;
2. Changchun Institute of Biological products, Changchun 130062, P. R. China

Received:1999-03-23 Online:2000-01-24 Published:2011-08-17
Supported by:
the National Natural Science Foundation of China

Abstract

Abstract: Trehalose can be determined by thin layer chromatography and high performance liquid chromatography.But it's difficult to distinguish trehalose from the similar bisaccharides.If the reaction of the reduced bisaccharides with phenyl hydrazine to form osazone is used, then the disturbance of the similar reduced bisaccharides can be rapidly and conveniently excluded, or via the ammoniated-reduced reaction, aliline can be specially bounded to the reduced hydroxyl of the bisaccharides.In that way trehalose can be accurately determined.

Key words: Trehalose, Thin layer chromatography, High performance liquid chromatography, Osazone, Ammoniated-reduced reaction

LIU Tie-min, LIU Ying. Studies on Determining Method of Trehalose[J]. Chemical Research in Chinese Universities, 2000, 16(1): 10-14.

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Studies on Determining Method of Trehalose

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