Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

doi:10.1007/s40242-015-5207-y

Chemical Research in Chinese Universities ›› 2015, Vol. 31 ›› Issue (6): 914-918.doi: 10.1007/s40242-015-5207-y

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Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

MA Yinghui¹, LIU Yuanyuan¹, WEI Mengying¹, SONG Fengrui², LIU Zhongying¹, PI Zifeng²

1. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

Received:2015-05-25 Revised:2015-07-06 Online:2015-11-01 Published:2015-11-24
Contact: LIU Zhongying, PI Zifeng E-mail:liuzy@jlu.edu.cn;mslab21@ciac.ac.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.81073040, 81274046, 81173507), the Jilin Provincial Science and Technology Agency-funded Projects, China(No.20150414040GH) and the National Basic Research Program of China(Nos.2011CB505300, 2011CB505305).

Abstract

Abstract:

The Caco-2 cells have been recognized as effective tools to be applied to imitating the drug absorption in human intestine for the transport of drug. Herein, Caco-2 cell monolayer model was used to study the transport of the ginsenoside compatibility with Veratrum nigrum in different proportions. A specific high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS) method was developed for the semiquantitative determination of ginsenoside in intestinal transport with Dioscin as an internal standard. For the Caco-2 model constructed, two influencing factors were investigated, including time and concentration. The results suggest that the absorption of ginsenoside Re, Rg1, Rb1, Rc, Rb2 and Rd are time- and concentration-dependent and the excretions of Rb1, Rc, Rb2 and Rd have a relatronship with some transport proteins. The bioavailability of the ginsenosides has reduced compared to the single Panax ginseng extract when compatibility with a certain amount of Veratrum nigrum.

Key words: High performance liquid chromatography-electrospray ionization-mass spectroscopy(HPLC-ESI-MS), Panax ginseng, Ginsenoside, Veratrum nigrum, Transport, Caco-2 cell monolayer model

MA Yinghui, LIU Yuanyuan, WEI Mengying, SONG Fengrui, LIU Zhongying, PI Zifeng. Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method[J]. Chemical Research in Chinese Universities, 2015, 31(6): 914-918.

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Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

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