MALDI Mass Spectrometry Analysis of Human Hemoglobin

Abstract

Abstract: Matrix-assisted laser desorption/ionisation(MALDI) mass spectra in matrices DHBs and ferulic acid have been taken and used to determine the molecular weight of one of the variants of human hemoglobin. Asingle laser operating at 355 nm was used to irradiate the sample droplet spots on a target for both desorption and ionisation. Ions were analyzed and detected by a reflectron time-of-flight mass spectrometer system. The mass values of α-chain and β-chain without heme group for a kind of human hemoglobin molecules were determined, which are 15275 Da and 16060Da respectively, with the accuracy of 0. 03%. The various factors influencing the resolution of mass spectrum were discussed.

Key words: Laser, Matrix, Hemoglobin

PENG Wei-xian. MALDI Mass Spectrometry Analysis of Human Hemoglobin[J]. Chemical Research in Chinese Universities, 1996, 12(1): 44-49.

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MALDI Mass Spectrometry Analysis of Human Hemoglobin

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