Studies on Surface Properties and Cell Adhesion Properties of BSA Modified DBM Scaffold

doi:10.1007/s40242-019-9020-x

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (4): 700-707.doi: 10.1007/s40242-019-9020-x

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Studies on Surface Properties and Cell Adhesion Properties of BSA Modified DBM Scaffold

SUN Mengjie¹, FENG Wei², WANG Bowei³, HAN Bing³, ZOU Jundong¹, YANG Chunying¹, LIU Zhihui¹

1. Stomatology Hospital of Jilin University, Changchun 130012, P. R. China;
2. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
3. The Second Hospital of Jilin University, Changchun 130022, P. R. China

Received:2019-01-18 Revised:2019-04-08 Online:2019-08-01 Published:2019-08-01
Contact: LIU Zhihui E-mail:liu-zh@jlu.edu.cn
Supported by:
Supported by the "Double Ten Project" of Changchun City, China(No.16ss12), the Scientific and Technological Achievements Transformation Project of Jilin Province, China(No.20170307024YY), the District(School, Institute) Cooperation Special Project of Changchun City, China(No.17DY024), the Key Technology Research and Development Projects of Jilin Province, China(No.20180201056YY), and the Science and Technology Development Medical Health Project of Jilin Province, China (No.20190304032YY).

Abstract

Abstract: As a scaffold material for bone tissue engineering, demineralized bone matrix(DBM) has such a limited ability to load cells and growth factors that the surface of the DBM scaffold was modified with bovine serum albumin(BSA) with different concentrations to improve the protein structure and physicochemical properties of the scaffold surface so as to enhance the adhesion of the cells. And the appropriate BSA concentration was explored. Compared with DBM, the scaffold with BSA coating had a smaller pore size and a lower porosity, also, the degradation rate was accelerated and the hydrophilic property was improved. Cells adhesion was observed inside the DBM scaffold before and after it had been modified, and the BSA modified scaffold had a good cell compatibility. When the concentration of BSA was 20 mg/mL, the adhesion ability of the cells to modified scaffold was significantly increased, and the cell proliferation was facilitated.

Key words: Scaffold, Demineralized bone matrix, Bovine serum albumin, Cell adhesion

SUN Mengjie, FENG Wei, WANG Bowei, HAN Bing, ZOU Jundong, YANG Chunying, LIU Zhihui. Studies on Surface Properties and Cell Adhesion Properties of BSA Modified DBM Scaffold[J]. Chemical Research in Chinese Universities, 2019, 35(4): 700-707.

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Studies on Surface Properties and Cell Adhesion Properties of BSA Modified DBM Scaffold

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