CRCU

Chemical Research in Chinese Universities ›› 2006, Vol. 22 ›› Issue (5): 612-616.doi:

• Articles • Previous Articles     Next Articles

Synthesis of Cell Adhesive Motif RGD Tripeptide by a Novel Chemical Method and Its Purification

WANG Hua1,2, ZHAO Mi-feng1, MA Cheng-yun2, JING Wei3, HUANG Yi-bing1, HOU Rui-zhen1, ZENG Hong-bin1, XU Li1 and ZHANG Xue-zhong1   

    1. Key Laboratory of Molecular Enzymology and Engineering, Jilin University, Changchun 130021, P. R. China;
    2. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    3. First Hospital of Jilin University, Changchun 130021, P. R. China
  • Received:2005-05-19 Revised:1900-01-01 Online:2006-09-25 Published:2006-09-25
  • Contact: ZHANG Xue-zhong E-mail:zhangxz@mail.jlu.edu.cn

Abstract: The cell adhesive motif RGD tripeptide was synthesized by using a novel chemical method. First, Gly-Asp(GD) was synthesized in two steps including the chloroacetylation of free L-aspartic acid and the ammonolysis of the chloroacetylated L-aspartic acid. The yield of chloroacetylated L-aspartic acid was 83.0%. For the ammonolysis of chloroacetylated L-aspartic acid, the yield of the ammonolyzed product was 92.3%. Second, the coupling between Arg and Gly-Asp was carried out by using the NCA method. The maximum yield of RGD was about 50% at 0 ℃ and pH=9.5. The prepared RGD tripeptide was confirmed by using amino acid component analysis and mass spectrographic analysis.

Key words: RGD tripeptide, Chemical synthesis, Purification