Preparation of a Hybrid Organic-inorganic Monolith for Extraction and Purification of Quercetin and Myricetin from Chamaecyparis obtusa

doi:10.1007/s40242-014-3394-6

Abstract

Abstract:

A new hybrid organic-inorganic monolithic cartridge was synthesized and used as the selective sorbent for the extraction and purification of quercetin and myricetin from Chamaecyparis obtusa via a solid-phase extraction method. The morphology of the monolithic material was examined by field emission-scanning electron microscopy and a Brunauer-Emmett-Teller(BET) test. The adsorption capacity of the obtained material for quercetin and myricetin was investigated by fitting the adsorption data to four different adsorption equations, of which the Langmuir-Freundlich isotherm was selected as the most suitable model. Under optimized conditions, good calibration curves were observed at nine concentrations ranged from 0.5 μg/mL to 100.0 μg/mL of quercetin and myricetin. The extraction recovery ranged from 74.5% to 84.6% and the inter-and intra-day relative standard deviations were <6%. This type of hybrid monolith has potential for the separation and purification of bioactive compounds from natural plant extracts.

Key words: Extraction, Purification, Adsorption isotherm, Hybrid organic-inorganic monolith, Quercetin, Myricetin

ZHU Tao, LEE Yuri, ROW Kyungho. Preparation of a Hybrid Organic-inorganic Monolith for Extraction and Purification of Quercetin and Myricetin from Chamaecyparis obtusa[J]. Chemical Research in Chinese Universities, 2014, 30(2): 216-221.

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