3D Printed Chitosan Bioink Loaded with Chrysin for Repairing Bone Defects

doi:10.1007/s40242-024-4216-0

Abstract

Abstract: This study aimed to investigate the potential of chrysin (C) to induce osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in vitro. Chitosan (CS) was dissolved by repeated freeze-thawing of alkali/urea solutions, followed by co-printing of nano-hydroxyapatite (HAP) powders with different concentrations of C solutions (5, 10, and 20 μmol/L) compounded into CS solution. Its rheological properties were modified with acetic acid/gelatin solution to construct C-CS-HAP (C-CS-H) composite scaffolds by extrusion printing technology. Physicochemical characterization showed that the four groups of scaffolds had regularly interconnected porous structures with pore diameters of about 450—600 μm, and the novel bioink exhibited shear-thinning properties, with the viscosity of the material decreasing in the shear rates range of 0.01—1000 s^-1 and thus good printability. The osteogenic differentiation ability of BMSCs was confirmed by the CCK-8 test, alkaline phosphatase (ALP) test, Alizarin Red staining (ARS) test, osteogenesis-related genes OCN, ALP, Runx 2, and bone morphogenetic protein-2 (BMP-2) test, which showed a significant promotion of the osteogenic differentiation ability of BMSCs with the increase of the content of C. The above results indicate the potential of C-CS-H composite scaffolds in osteogenesis.

Key words: Chrysin, Chitosan, Bioscaffold, 3D printing, Hydroxyapatite

ZHANG Hao, TANG Wenshuo, SUI Xin, LI Lingfeng, ZHANG Hongpu, LI Yuran, ZHANG Xueyi, LIU Zhihui, WANG Bowei. 3D Printed Chitosan Bioink Loaded with Chrysin for Repairing Bone Defects[J]. Chemical Research in Chinese Universities, 2025, 41(1): 155-164.

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