Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids

doi:10.1007/s40242-019-8283-6

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (3): 504-508.doi: 10.1007/s40242-019-8283-6

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Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids

CAO Jing^1,2, WANG Miao^1,2, CHEN Weihua³, SHE Yongxin^1,2, WANG Jing^1,2,4, WANG Fengzhong³, LAO Shuibing⁴

1. Institute of Quality Standardization & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China;
2. Key Laboratory of Agrofood Safety and Quality(Beijing), Ministry of Agriculture, Beijing 100193, P. R. China;
3. Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China;
4. Agro-products Quality Safety and Testing Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 53003, P. R. China

Received:2018-09-10 Revised:2019-01-18 Online:2019-06-01 Published:2019-04-26
Contact: WANG Miao, WANG Jing E-mail:wm0510@126.com;w_jing@126.com
Supported by:
Supported by the National Natural Science Foundation of China(Nos.31501571, 31772071), the Fund of Agriculture Research System, China(No.CARS-05-05A-03), the Innovation-driven Development Project of Guangxi Province, China (No.AA17204043-2), and the Science and Technology Project of Tibet Autonomous Region, China(No.XZ201901NA04).

Abstract

Abstract: A novel artificial esterase based on chitosan and amino acids was synthesized in the present study. The Fmoc-His and Glu were linked to chitosan by active ester method(AEM). The hydroxide radical in chitosan, imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase. Gel microspheres were coated with a protective layer and a supporting layer by self-assembly construction function in carboxymethylcellulose sodium(CMCS) solution. As for catalytic activity, chitosan-His-Glu was found to be more efficient than chitosan-His and chitosan-Glu in mimicking the core catalytic sites of natural esterase, and the best ratio(mass ratio) of chitosan-His-Glu:CMCS was 1:3. Furthermore, metal ions, such as Ca²⁺, Mg²⁺, Fe²⁺, etc., were able to improve the catalytic efficiency of artificial esterase. And the Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation.

Key words: Artificial enzyme, Esterase, Chitosan

CAO Jing, WANG Miao, CHEN Weihua, SHE Yongxin, WANG Jing, WANG Fengzhong, LAO Shuibing. Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids[J]. Chemical Research in Chinese Universities, 2019, 35(3): 504-508.

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Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids

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