QCM and EC-SPR Studies of Cytochrome cSelf-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (6): 1061-1065.

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QCM and EC-SPR Studies of Cytochrome cSelf-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles

WANG Yan-yan, JIANG Yan-xia, ZHOU You-chen, LI Yan-yan, MA Zhi-fang, SUN Shi-gang

State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China

Received:2012-02-16 Revised:2012-03-28 Online:2012-11-25 Published:2012-11-09
Contact: JIANG Yan-xia E-mail:yxjiang@xmu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.20833005, 20873116, 60936003 and 21021002) and the Fund of the State Key Laboratory of Superamolecular Structure and Materials, Jilin University, China(No.200910).

Abstract

Abstract:

Quartz crystal microbalance(QCM) and cyclic voltammetry(CV) were used to characterize the monolayer of cytochrome c(Cyt c), which was adsorbed on gold film modified with alkanethiol mixed monolayer. A direct comparison of protein surface coverages calculated from QCM and cyclic voltammetric measurements illustrates that the ratio of the electroactive Cyt c to the total surface-confined Cyt cis 34%, which suggests that the orientation is a main factor affecting the electroactivity of Cyt c. Moreover, surface plasmon resonance(SPR) measurement combined with CV “in situ” was used to investigate the conformational change of Cyt c in the redox process. Besides, Au nanoparticles(Au NPs) were adsorbed on the surface of Cyt c. The result indicates that Au NPs promote electron transfer between Cyt c and the gold electrode, and SPR result suggests Au NPs enhance SPR signal.

Key words: Cytochrome c, Gold film, Self-assembly, Electrochemical-surface plasmon resonance(EC-SPR)

WANG Yan-yan, JIANG Yan-xia, ZHOU You-chen, LI Yan-yan, MA Zhi-fang, SUN Shi-gang. QCM and EC-SPR Studies of Cytochrome cSelf-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles[J]. Chemical Research in Chinese Universities, 2012, 28(6): 1061-1065.

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QCM and EC-SPR Studies of Cytochrome cSelf-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles

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