CRCU

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (4): 614-619.

• Articles • Previous Articles     Next Articles

Luminescence Studies on Interaction of an Isoquinoline Alkaloid with Calf Thymus DNA in Aqueous Medium and on Solid Substrate

LI Jun-fen1, SHI Wen-tao2, HAN Xiu-ying1, DONG Chuan2, CHOI Martin M. F. 3   

  1. 1. School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, P. R. China;
    2. Research Center of Environmental Science and Engineering, Shanxi University, Taiyuan 030006, P. R. China;
    3. Department of Chemistry, Hong Kong Baptist University, Hong Kong, P. R. China
  • Received:2011-08-17 Revised:2011-12-27 Online:2012-07-25 Published:2012-07-25
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.21175086, 21105060), the Natural Science Foundation of Shanxi Province, China(No.2010011013-1) and the Research Project Supported by Shanxi Scholarship Council, China.

PDF (PC)

Cited

3

Abstract: The interaction of calf thymus DNA(ctDNA) with berberine(Ber) was systematically investigated via UV-Vis, fluorescence and phosphorescence spectroscopies in aqueous solution and on solid substrate. The very weak fluorescence intensity of Ber at 525 nm in aqueous solution can be greatly enhanced by ctDNA. The UV-Vis spectrum shows that Ber could bind with DNA by intercalation. In addition, strong fluorescence of Ber alone could be observed on filter paper substrate and can be quenched by ctDNA effectively, and the increase of fluorescence polarization of Ber on the filter paper implies the intercalation binding. Ber emits phosphorescence at 619 nm in the presence of thallium(I) acetate. The lifetime of Ber increased from 38.4 ms to 43.4 ms with the increase of ctDNA. The increase of lifetime is possibly attributed to the increase of rigidity of Ber after its intercalating into the ctDNA base pairs. This explanation is also augmented by the anionic quenching results. Ber behaves as a typical intercalating agent into ctDNA.The intrinsic binding constant between Ber and ctDNA is (1.84?0.12)?104 L/mol and n=0.16.

Key words: Berberine, Calf thymus DNA, UV-Vis spectroscopy, Luminescence, Interaction