Analysis of Low-polar Ginsenosides in Steamed Panax Ginseng at High-temperature by HPLC-ESI-MS/MS

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (1): 31-36.

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Analysis of Low-polar Ginsenosides in Steamed Panax Ginseng at High-temperature by HPLC-ESI-MS/MS

ZHANG Yu-chi^1,2, PI Zi-feng^1*, LIU Chun-ming², SONG Feng-rui^1*, LIU Zhi-qiang¹, LIU Shu-ying¹

1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
2. Central Laboratory, Changchun Normal University, Changchun 130032, P. R. China

Received:2011-04-01 Revised:2011-06-02 Online:2012-01-25 Published:2011-12-27
Contact: Zifeng Pi E-mail:mslab21@ciac.jl.cn; songfr@ciac.jl.cn
Supported by:
Supported by the Key Project of Jilin Provincial Science and Technology Department, China(No.20090908), the Project of Changchun Science and Technology Bureau, China(No.2008256) and the Special Cooperative Project for Hi-tech Industrialization of Jilin Provincial Science and Chinese Academy of Sciences, China(No.2009SYHZ0026).

Abstract

Abstract: A high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrometry( HPLC-ESI-MS/MS) method was developed for the analysis and identification of ginsenosides in the extracts of raw Panax ginseng(RPG) and steamed Panax ginseng at high temperatures(SPGHT). A total of 25 ginsenosides were extracted include of which 10 low-polar ginsenosides, such as ginsenosides F4, Rk3, Rh4, 20S-Rg3, 20R-Rg3 and so on, were identified according to their HPLC retention time and MS/MS data. The results indicated that the low polar ginsenosides were seldom found in RPG. For the exploration of the transformation pattern of the ginsenosides in steam processing, the standards of ginsenosides Re, Rg1, Rb1, Rc, Rb2, Rb3 and Rd were selected and hydrolyzed at a temperature of 120 ℃. The results show that these polar ginsenosides can be converted to low-polar ginsenosides such as Rg2, Rg6, F4, Rk3 and Rg5 by hydrolyzing the sugar chains.

Key words: Ginsenoside, Hydrolysis, Steamed Panax ginseng, High performance liquid chromatography coupled with electrospray ionization-tandem mass spectrometry(HPLC-ESI-MS/MS)

ZHANG Yu-chi, PI Zi-feng, LIU Chun-ming, SONG Feng-rui, LIU Zhi-qiang, LIU Shu-ying. Analysis of Low-polar Ginsenosides in Steamed Panax Ginseng at High-temperature by HPLC-ESI-MS/MS[J]. Chemical Research in Chinese Universities, 2012, 28(1): 31-36.

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Analysis of Low-polar Ginsenosides in Steamed Panax Ginseng at High-temperature by HPLC-ESI-MS/MS

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