Determination of Amino Acids in Panax notoginseng by Microwave Hydrolysis and Derivatization Coupled with Capillary Zone Electrophoresis Detection

doi:10.1007/s40242-013-2325-2

Abstract

Abstract:

The microwave hydrolysis and derivatization coupled with capillary electrophoresis detection were developed for the separation and determination of the amino acids in Panax notoginseng.The experimental conditions for the microwave hydrolysis and derivatization were examined and optimized. Several parameters of capillary electrophoresis, such as pH value of background electrolyte, borate concentration and applied voltage were optimized. Under the selected conditions, 11 amino acids were completely separated. The real sample was analyzed and the results were satisfactory. Compared with that of conventional heat hydrolysis and derivatization, the analytical time of this method was significantly shortened.

Key words: Microwave hydrolysis, Derivatization, Amino acid, Capillary electrophoresis, Panax notoginseng, 2,4-Dinitrofluorobenene

LI Xiao-tian, ZHAO Ya-jing, JIANG Cheng-fei, ZHANG Han-qi, YU Ai-min. Determination of Amino Acids in Panax notoginseng by Microwave Hydrolysis and Derivatization Coupled with Capillary Zone Electrophoresis Detection[J]. Chemical Research in Chinese Universities, 2013, 29(3): 434-438.

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