Facial Preparation of Magnetic Lipase as Efficient Biocatalyst to Resolute Esters Enantioselectively

doi:10.1007/s40242-015-5209-9

Chemical Research in Chinese Universities ›› 2015, Vol. 31 ›› Issue (6): 997-1002.doi: 10.1007/s40242-015-5209-9

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Facial Preparation of Magnetic Lipase as Efficient Biocatalyst to Resolute Esters Enantioselectively

ZHANG Yanmei, QUAN Chunshan, LIU Baoquan, FAN Shengdi

College of Life Science, Dalian Nationalities University, Dalian 116600, P. R. China

Received:2015-05-26 Revised:2015-08-16 Online:2015-11-01 Published:2015-09-14
Contact: ZHANG Yanmei E-mail:ymzhang@dlnu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21203017), the Open Fund of State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences(No.N-11-3), the Program for Liaoning Excellent Talents in University, China and the Fundamental Research Funds for the Central Universities, China(No.DC201502020304).

Abstract

Abstract:

Magnetic lipase was prepared by a facial and cost-effective method. Lipase from Pseudomonase cepacia was covalently linked to Fe₃O₄ nanoparticles, which were produced by co-precipitating Fe²⁺ and Fe³⁺ ions in ammonia solution and subsequent coating with 3,4-dihydroxylaldehyde. Magnetic Fe₃O₄ nanoparticles modified with 3,4-dihydroxylaldehyde bonded lipase efficiently(47 mg/g). The resulting magnetic-lipase is active(0.68 μmol·min^-1·mg^-1) and enantioselective(e.e. of the product >98%) in the hydrolysis of racemic 1-phenylethyl acetate. Furthermore, as a heterogeneous catalyst, the magnetic-lipase can be magnetically recycled, and a retained activity of 48% was obtained even after 6 cycles.

Key words: Covalent attachment, 3,4-Dihydroxylaldehyde, Enantioselective hydrolysis, Lipases from Pseudomonase cepacia, Fe₃O₄ nanoparticle, Recycle

ZHANG Yanmei, QUAN Chunshan, LIU Baoquan, FAN Shengdi. Facial Preparation of Magnetic Lipase as Efficient Biocatalyst to Resolute Esters Enantioselectively[J]. Chemical Research in Chinese Universities, 2015, 31(6): 997-1002.

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Facial Preparation of Magnetic Lipase as Efficient Biocatalyst to Resolute Esters Enantioselectively

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