CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (4): 584-588.

• Articles • Previous Articles     Next Articles

Extraction and Identification of Ginsenoside Re and Its Effects and Mechanism of Protecting Acute Renal Ischemia-reperfusion Injury in Rats

GU Xin-quan1, CHEN Yan-ping2, HU Ting-ting3, LU Xiu-hua3, LI Xi-qian1, DU Xiao-hui3, CAO Xia3*, WANG Wei-hua1* and XU Zhong-gao4   

  1. 1. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    2. College of Chemistry, Jilin University, Changchun 130012, P. R. China;
    3. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
    4. The First Hospital of Jilin University, Changchun 130021, P. R. China
  • Received:2011-03-21 Revised:2011-04-22 Online:2011-07-25 Published:2011-06-29
  • Contact: CAO Xia;WANG Wei-hua E-mail:caoxia@jlu.edu.cn; wei@uro.med.tohoku.ac.jp
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.30701128) and the Fund of Jilin Provincial Science and Technology Department, China(No.200705377).

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Abstract: This paper studies the extraction and identification of ginsenoside Re from ginseng fruits and investigates the effect and mechanism of ginsenoside Re of protecting acute renal ischemia-reperfusion injury in rats. Having been smashed, the ginseng fruits were ultrasonically extracted twice with 95% ethanol for 30 min each. Having been   concentrated, the solution was dissolved with distilled water and separated by two-column chromatography, of  which one was packed with macroporous resin D4020, and the other was packed with macroporous resin D941. The raw product was dissolved with methanol and was purified by elution on a Si gel column, finally ginsenoside Re was        obtained. The structure of the ginsenoside Re was analyzed by the thin-layer chromatography and NMR methods, and HPLC was carried for the content determination. The model of acute renal ischemia-reperfusion injury in rats    was established after ischemia for 1 h and reperfusion for 1 h or 24 h, serum SOD(superoxide dismutase), MDA(malondialdehyde) and plasma TXB2(thromboxane B2) and 6-keto-PGF1α were detected. The results show that it has accurately, fast, convenient merits and so on. Ginsenoside Re has a protective effect on acute renal ischemia-reperfusion injury in rats, the mechanism may be related to improving the imbalance of thromboxane A2(TXA2)/prostacyclin(PGI2) and inhibiting lipid peroxidation reaction.

Key words: Ginsenoside Re, Extraction, Identification, Kidney, Ischemia-reperfusion injury