Studies on New Steroidal Saponins from Allii macrostemonis Bulbus and Their Antitumor Activities

doi:10.1007/s40242-018-7213-3

Chemical Research in Chinese Universities ›› 2018, Vol. 34 ›› Issue (1): 61-66.doi: 10.1007/s40242-018-7213-3

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Studies on New Steroidal Saponins from Allii macrostemonis Bulbus and Their Antitumor Activities

YAO Hua¹, WANG Tong², WU Qian², LIU Ying², LI Peng², LI Xuwen², JIN Yongri²

1. Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
2. College of Chemistry, Jilin University, Changchun 130012, P. R. China

Received:2017-06-16 Online:2018-02-01 Published:2018-01-20
Contact: JIN Yongri,E-mail:jinyr@jlu.edu.cn E-mail:jinyr@jlu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.31201295).

Abstract

Abstract: Two new steroidal saponins(2, 3) and two known compounds(1, 4) were isolated from 75% ethanol extract of Allii macrostemonis bulbus by various spectral methods. The two new steroidal saponins were respectively elucidated as (25S)-26-O-β-D-glucopyranosyl-5α-furostanol-2α,3β,22,26-tetrol-3-O-β-D-glucopyranosyl(1→2)-[β-D- glucopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside(2) and 26-O-β-D-glucopyranosyl-5α- furostanol-25(27)-ene-3β,22,26-tetrol-3-O-β-D-glucopyranosyl(1→2)-[β-D-glucopyranosyl(1→3)]-β-D-glucopyranosyl- (1→4)-β-D-galactopyranoside(3). Compound 4 was obtained from this medical plant for the first time. The activity experiments of proliferation inhibition on tumor cells were performed by cell counting kit-8(CCK-8) method for compound 2(25S-configuration) and its isomer(compound 1, 25R-configuration). The results show that compounds 1 and 2 have weak inhibition on lung cancer A549 cells, melanoma A375 cells and breast cancer MCF-7 cells as well as certain inhibitory effect on liver cancer HepG2 cells, and the inhibitory effect of compound 1 is stronger than that of compound 2.

Key words: Allii macrostemonis bulbus, Steroidal saponin, Structure identification, Proliferation inhibition, Cell counting kit-8(CCK-8) method

YAO Hua, WANG Tong, WU Qian, LIU Ying, LI Peng, LI Xuwen, JIN Yongri. Studies on New Steroidal Saponins from Allii macrostemonis Bulbus and Their Antitumor Activities[J]. Chemical Research in Chinese Universities, 2018, 34(1): 61-66.

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