Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography

doi:10.1007/s40242-019-8216-4

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (2): 209-215.doi: 10.1007/s40242-019-8216-4

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Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography

GUO Donggang^1,2, NI Liang¹, WANG Liang², SHAO Li²

1. School of the Environment and Safety Engineering, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P. R. China;
2. Key Laboratory of Preparation and Application of Environmental Friendly Materials, Jilin Normal University, Ministry of Education, Changchun 130103, P. R. China

Received:2018-07-02 Revised:2018-08-25 Online:2019-04-01 Published:2019-04-30
Contact: NI Liang E-mail:niliang@ujs.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21407058).

Abstract

Abstract: A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can't effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and NaH₂PO₄ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of NaH₂PO₄, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30℃ by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31.

Key words: Binary small molecule alcohol aqueous two-phase system, Partition behavior, Chloramphenicol, Extraction efficiency

GUO Donggang, NI Liang, WANG Liang, SHAO Li. Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography[J]. Chemical Research in Chinese Universities, 2019, 35(2): 209-215.

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Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography

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