Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase

Abstract

Abstract: The S-adenosylmethionine synthetase (SAM synthetase) is responsible for in vivo synthesis of S-adenosylmethionine(SAM), which is a kind of biologically active molecules distributed in all body tissues and fluids and involved in a number of biochemical reactions.In this study, a cDNA containing the coding sequence for rat liver SAM synthetase was cloned into the prokaryotic expression vector pQE30 and expressed in E.coli M15.A major band corresponding to a protein of 48 kDa was detected on SDS-PAGE.The protein was distributed in both the soluble fraction and the insoluble fraction.In soluble fractions the protein was fully active.

Key words: SAM, SAM synthetase, Cloning, Expression, HPLC

CUI Wei, HUANG Lei, LI Wei, WANG Li-ping. Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase[J]. Chemical Research in Chinese Universities, 2006, 22(2): 236-238.

[1]	LIU Qianyu, DU Zhenwu, ZHU He, ZHAO Tianyu, PAN Ying. Comprehensive Analysis of Differential Gene Expression Profile via RNA Sequencing in the Human Ovarian Cancer SKOV3 Cells Treated with Simvastatin [J]. Chemical Research in Chinese Universities, 2022, 38(2): 632-638.
[2]	YANG Jinling, DONG Wenhua, CHEN Huiqin, KONG Fandong, WANG Jun, MEI Wenli, DAI Haofu. Qualitative and Quantitative Analysis of Flidersiachromones in Three Agarwood Samples by HPLC-MS/MS [J]. Chemical Research in Chinese Universities, 2018, 34(3): 389-396.
[3]	WU Qian, JIN Yongri, YAO Hua, LIU Ying, LI Peng, LI Xuwen. Two New Baccharane Glycosides from Seeds of Impatientis balsamina L. with in vitro Growth Inhibitory Activity in Human Cancer A375 Cells [J]. Chemical Research in Chinese Universities, 2017, 33(5): 753-757.
[4]	ZHU Dan, JIN Wei, YU Bingwen, YING Yangwei, YU Haixiang, SHAN Jin, YAN Yuwei, XU Chen, WANG Baolai, JIN Qinhan. Development of a Novel Kilowatt Microwave Plasma Torch Source for Atomic Emission Spectrometry [J]. Chemical Research in Chinese Universities, 2017, 33(5): 709-713.
[5]	CUI Xueqin, MA Zhongxiao, BAI Lu, WU Yong, GUO Sen, LIU Qingchao, ZHANG Li, HO Chi-Tang, BAI Naisheng. Phytochemical Analysis of Ziziphus jujuba Leaves in Six Cultivars at the Whole Life Stage by High Performance Liquid Chromatography [J]. Chemical Research in Chinese Universities, 2017, 33(5): 702-708.
[6]	BIAN Min, TIAN Ling, YAO Cheng. An Improved Method of Simultaneous Determination of Four Bioactive Compounds in Evodiae Fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography [J]. Chemical Research in Chinese Universities, 2017, 33(4): 552-558.
[7]	WANG Tongtong, SONG Yinqing, WANG Min, TANG Xiaoyan, ZHOU Jian, YANG Mengrui. Total Synthesis and High Performance Liquid Chromatography Analysis of Phenylethanolamine A [J]. Chemical Research in Chinese Universities, 2017, 33(4): 598-602.
[8]	TIAN Xiao, YANG Meiqi, GUO Sen, LIU Qingchao, ZHANG Li, HO Chi-Tang, BAI Naisheng. Quantitative Analysis and Chemical Fingerprint Similarity for Quality Control of the Seeds of Paeonia suffruticosa Andr. by HPLC [J]. Chemical Research in Chinese Universities, 2017, 33(4): 546-551.
[9]	Muhammad ASGHAR, Mohammad YAQOOB, Abdul NABI. Chemiluminescent Determination of Cyromazine in Milk Samples Using Copper (III) Chelate-Triton X-100 by Flow Injection Analysis [J]. Chemical Research in Chinese Universities, 2017, 33(3): 354-359.
[10]	UANSIRI Sutthirak, VICHAPONG Jitlada, KANCHANAMAYOON Wanna. Ultrasound-assisted Low Density Solvent Based Dispersive Liquid-liquid Microextraction for Determination of Phthalate Esters in Bottled Water Samples [J]. Chemical Research in Chinese Universities, 2016, 32(2): 178-183.
[11]	FU Yuqin, LI Luhua, WANG Hui, JIANG Yuan, LIU Huijing, CUI Xiyan, WANG Piwu, LÜ Changli. Silica Nanoparticles-mediated Stable Genetic Transformation in Nicotiana tabacum [J]. Chemical Research in Chinese Universities, 2015, 31(6): 976-981.
[12]	MA Yinghui, LIU Yuanyuan, WEI Mengying, SONG Fengrui, LIU Zhongying, PI Zifeng. Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method [J]. Chemical Research in Chinese Universities, 2015, 31(6): 914-918.
[13]	WANG Hui, WANG Jiayuan, WU Xiaopeng, SHEN Haiyan, LUO Ying, DAI Haofu, MEI Wenli. HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana [J]. Chemical Research in Chinese Universities, 2015, 31(1): 38-43.
[14]	HAN Jing, WU Xi, CAI Wensheng, SHAO Xueguang. Rapid Determination of Amino Acids in Ginseng by High Performance Liquid Chromatography and Chemometric Resolution [J]. Chemical Research in Chinese Universities, 2014, 30(4): 578-581.
[15]	REN Li, LIU Yang, YU Guihua, GAO Yuan, LIU Xin, WANG Bo, DENG Xiaonan, CHENG Maosheng. Synthesis and Tumor Cytotoxicity of Novel N-Substituted Glucosamine-bearing Oleanolic Acid Derivatives [J]. Chemical Research in Chinese Universities, 2014, 30(4): 639-643.

Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments