CRCU

Chemical Research in Chinese Universities ›› 2018, Vol. 34 ›› Issue (3): 389-396.doi: 10.1007/s40242-018-7273-4

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Qualitative and Quantitative Analysis of Flidersiachromones in Three Agarwood Samples by HPLC-MS/MS

YANG Jinling, DONG Wenhua, CHEN Huiqin, KONG Fandong, WANG Jun, MEI Wenli, DAI Haofu   

  1. Hainan Engineering Research Center of Agarwood, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P. R. China
  • Received:2017-08-21 Revised:2018-01-24 Online:2018-06-01 Published:2018-03-26
  • Contact: 10.1007/s40242-018-7273-4 E-mail:meiwenli@itbb.org.cn;daihaofu@itbb.org.cn
  • Supported by:
    Supported by the Project of the Innovative Research Team Grant of the Natural Science Foundation of Hainan Province, China (No.2017CXTD020), the Project of the China Agriculture Research System(No.CARS-21) and the Central Public-interest Scientific Institution Basal Research Fund for the Chinese Academy of Tropical Agricultural Sciences(Nos.17CXTD-15, 1630052016008).

Abstract: HPLC/ESI/MS2 was used in the qualitative and quantitative analysis of flidersiachromones(FTPECs) in three agarwood samples of Aquilaria sinensis, including an artificial holing agarwood, a wild agarwood, and a high quality agarwood named "Qi Nan". From these three agarwood samples, totally forty-six FTPECs were identified, and twenty-five of which were tentatively identified by the analysis of MS fragmentation ions, the others were indentified via comparing the retention time and MS fragmentation ions of them with those of the reference compounds. Besides, quantitative determination of five characteristic FTPECs(F14, F16 and F18-F20) in the three agarwoods was carried out, and it was found that the absolute content of each compound of these five FTPECs showed positive correlation with the quality of agarwood samples. Due to the wide distribution of four of these FTPECs(F16 and F18-F20) in the chemical constituents of agarwood, it is suggested that the total absolute content of these four FTPECs may be set as one of basis for agarwood quality evaluation.

Key words: Agarwood, Aquilaria sinensis, HPLC/DAD/ESI/MS/MS, Flidersiachromone, Quantitative determination