Abstract: A new chemically modified electrode(CME) immobilized on the surface of multi-wall carbon nanotubes functionalized with carboxylic groups was fabricated.The results indicate that the CME exhibits efficiently electrocatalytic oxidation of 6-mercaptopurine(6-MP).The CME can be used as the working electrode in the liquid chromatography for the determination of 6-MP.The peak current of 6-MP is linearly changed with its concentration ranging from 4.0×10^-7 to 1.0×10^-4 mol/L with the calculated detection limit (S/N=3) of 2.0×10^-7 mol/L.Coupled with microdialysis sampling, the method has been successfully applied to assessing the content of 6-MP in rat blood.

Key words: 6-Mercaptopurine, Multi-wall carbon nanotube, Chemical modified electrode, HPLC, Microdialysis sampling