Chemical Research in Chinese Universities ›› 2001, Vol. 17 ›› Issue (4): 368-373.

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Studies on the Basic Conformation of Lysozyme by Electrospray Ionization-Mass Spectrometry (ESI- MS)

GU Sheng, SHEN Jin-can, ZHUANG Zhi-xia, WANG Xiao-ru   

  1. Key Laboratory of Analytical Science of Ministry of Education, Department of Chemistry, Xiamen University, Xiamen 361005, P. R. China
  • Received:2001-09-10 Online:2001-12-24 Published:2011-08-04
  • Supported by:

    Supported by the National Natural Science Foundation of China.

Abstract: In the present work, 2-mercaptoethanol and dithiothreitol were used to hydrogenise the internal disulfide bond in lysozyme.The experimental results indicate that the charge distribution of the proteins are different in the reaction process.From the calculated molecular weight, the reduction process of the disulfide bond in the molecules can be described, and the number of the disulfide bonds in the molecule can also be determined.

Key words: Protein, Disulfide, Electrospray ionization, Mass spectrometry