Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin

Chemical Research in Chinese Universities ›› 1999, Vol. 15 ›› Issue (4): 333-336.

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Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin

GOU Xiao-jun¹, LI Xiang-hui¹, DING Tian-bing¹, WANG Yong-ting¹, CHEN Huai-yong¹, HUANG Cheng-fang¹, DONG Qing-chu¹, LI Qing-shan¹, WANG Yan-zhong², ZHAO Lu-lu³

1. College of Life Science, Jilin University, Changchun 130023, P. R. China;
2. Medical Testing Depa rtment, People's Hospital of Jilin Province, Changchun 130021, P. R. China;
3. Changchun Higher College of Medical Sciences, Changchun 130031, P. R. China

Received:1999-02-08 Online:1999-12-24 Published:2011-08-17

Abstract

Abstract: The Rana temporaria chensinensis is an amphibian distributing mainly over the north of China. Its skin gland contains a variety of peptides, including antibacterial peptides. One of them, component 1, has been purified from the dried skin of R.temporaria chensinesis using a five-step procedure. Purified component 1 has a molecular weight of 5700 and its amino acid composition shows that it is devoid of disulfide bridges but rich in histine and glycine residues. Moreover, it has been found that it has a broad spectrum of antibacterial activity for Pseuctomonas aeruginosa, Escherichia coli, Bacillus sublilis, S. faccalis and Proteus vulgaris .

Key words: Antibacterial activity, Peptide, Dried skin

GOU Xiao-jun, LI Xiang-hui, DING Tian-bing, WANG Yong-ting, CHEN Huai-yong, HUANG Cheng-fang, DONG Qing-chu, LI Qing-shan, WANG Yan-zhong, ZHAO Lu-lu . Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin[J]. Chemical Research in Chinese Universities, 1999, 15(4): 333-336.

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Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin

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