A Polysaccharide-based Hydrogel and PLGA Microspheres for Sustained P24 Peptide Delivery: An In vitro and In vivo Study Based on Osteogenic Capability

doi:10.1007/s40242-019-9177-3

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (5): 908-915.doi: 10.1007/s40242-019-9177-3

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A Polysaccharide-based Hydrogel and PLGA Microspheres for Sustained P24 Peptide Delivery: An In vitro and In vivo Study Based on Osteogenic Capability

CAI Qing¹, QIAO Chunyan¹, NING Jun², DING Xinxin¹, WANG Haoyang¹, ZHOU Yanmin¹

1. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School of Stomotology, Jilin University, Changchun 130021, P. R. China;
2. The First Hospital of Jilin University, Changchun 130021, P. R. China

Received:2019-06-25 Online:2019-10-01 Published:2019-09-24
Contact: ZHOU Yanmin E-mail:zhouym@jlu.edu.cn
Supported by:
Supported by the Research Fund of the Education Department of Jilin Province, China(No.JJKH20190202KJ), the Research Fund of the Development and Reform Commission of Jilin Province, China(No.2019C051-8), the International Cooperation Project of the Science and Technology Department of Jilin Province, China(No.2018KJT084) and the Health and Family Planning Research Project of Jilin Province, China(No.2016Q019).

Abstract

Abstract: A bone morphogenetic protein-2(BMP-2) derived synthetic oligopeptide, S[PO₄]KIPKASSVPTELSAI-STLYLDDD(P24), has shown great potential for facilitating bone regeneration. However, P24 cannot be directly used onto bone defects, while a continuous sustained delivery of P24 may lead to a better formation of bone tissue. Based on this issue, we have developed a sustained delivery system incorporating P24-loaded poly(lactide-co-glycolide) (PLGA) microspheres and nano-hydroxyapatite(n-HA) into the composite hydrogel. The P24-contained compound material was characterized with NMR, FTIR and SEM to demonstrate the formation of compound structure contai-ning P24, PLGA and n-HA. A continuous drug release of P24 was observed for over 60 d that evidently enhanced the efficiency in promoting the proliferation of MC3T3-E1 cells and the secrete of alkaline phosphatase(ALP) in vitro. Moreover, the osteoinduction effect of the hydrogel system with P24 peptide microspheres was demonstrated in vivo and manifested by the result of immunohistochemistry. This novel injectable composite hydrogel is expected to be applied to improving the bone defect treatment in bone tissue engineering.

Key words: BMP-2 derived peptide, Hydrogel, PLGA microsphere, Osteogenesis, Tissue engineering

CAI Qing, QIAO Chunyan, NING Jun, DING Xinxin, WANG Haoyang, ZHOU Yanmin. A Polysaccharide-based Hydrogel and PLGA Microspheres for Sustained P24 Peptide Delivery: An In vitro and In vivo Study Based on Osteogenic Capability[J]. Chemical Research in Chinese Universities, 2019, 35(5): 908-915.

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A Polysaccharide-based Hydrogel and PLGA Microspheres for Sustained P24 Peptide Delivery: An In vitro and In vivo Study Based on Osteogenic Capability

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