Chemical Research in Chinese Universities ›› 1998, Vol. 14 ›› Issue (4): 420-422.

• Articles • Previous Articles     Next Articles

A Simple Method Designed for the Separation of Biotinylated Ligate from Free Biotin

LI Tian-yu, ZHOU Chang-cheng, FANG Rui, QI Jie, ZHOU Hui, LI Wei   

  1. Department of Life Science, Jilin University, Changchun 130023
  • Received:1997-09-17 Online:1998-12-24 Published:2011-08-17

Abstract: Considering the chemical characteristics of the biotinylate reagent,we designed a method for the separation of biotinylatedlig ate from residual biot inylate reagent with equal efficiency to ultr afiltration.This metho disespecially fit for the separation of biotinylating small molecules.

Key words: Biotinylated Ligate, Biotin, Protein