Development of BCPDA-Eu3+-BAS Labeled Hepatitis B Surface Antibodies

doi:10.1007/s40242-018-7295-y

Chemical Research in Chinese Universities ›› 2018, Vol. 34 ›› Issue (1): 24-27.doi: 10.1007/s40242-018-7295-y

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Development of BCPDA-Eu³⁺-BAS Labeled Hepatitis B Surface Antibodies

TAN Wenjia¹, SUN Zhixia¹, XUAN Lili¹, LI Junling², XIE Wenbing², HE Chengyan¹, PAN Lihua²

1. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

Received:2017-09-06 Online:2018-02-01 Published:2018-01-20
Contact: HE Chengyan,E-mail:chengyanhe469@vip.sina.com;PAN Lihua,E-mail:lihuap@ciac.ac.cn E-mail:chengyanhe469@vip.sina.com;lihuap@ciac.ac.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.81572082) and the Natural Science Foundation of Jilin Province, China(Nos.2011713, 20150414015GH, 20150204010YY).

Abstract

Abstract: The effect of the chelating agent 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) labeled hepatitis B surface antibodies(HBsAb) on time-resolved fluoroimmunoassays(TRFIA) was investigated. The labeling detection method was established. The biotin-streptavidin(BAS) system was introduced, and the multi-stage amplification effect of the BAS system was analyzed. It is found that the BCPDA-Eu³⁺-HBsAb-BAS fluorescent marker can emit strong fluorescence. Compared to BCPDA-Eu³⁺-HBsAb, BCPDA-Eu³⁺-HBsAb-BAS demonstrates an enhanced fluorescence intensity by over 10 times. This study provides a guidance for the next gene- ration non-radio immunoassays in clinical diagnosis.

Key words: Time-resolved fluorescence immunoassay, Biotin, Streptavidin

TAN Wenjia, SUN Zhixia, XUAN Lili, LI Junling, XIE Wenbing, HE Chengyan, PAN Lihua. Development of BCPDA-Eu³⁺-BAS Labeled Hepatitis B Surface Antibodies[J]. Chemical Research in Chinese Universities, 2018, 34(1): 24-27.

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Development of BCPDA-Eu³⁺-BAS Labeled Hepatitis B Surface Antibodies

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