Abstract: A glucose oxidase (GOD) electrode with tetrathiafulvalene(TTF) as an elec-tron transfer mediator is described.By means of Nafion, TTFwas modified on aglassy carbon(GC) electrode surface, and glucose oxidase was then immobilized onthe TTF-Na fion film, forming a GOD-TTF-Na fion enzyme electrode.Characteris-tics of the sensor are shown by cyclic voltammetry and constant potential measure-ments.The sensor responds rapidly to glucose, the time required to reach 95% of the steady-state current is less than 60s.The sensor displays a sensitive catalyticcurrent response to glucose and can be operated in a potential range in which theoxidation of common interfering species, such as ascorbic acid and uric acid, doesnot occur.The sensor is stable for more than 30 days at 4℃ and plasma glucose assays were correlated with spectrophotometry(n=10, r=0.995).

Key words: Tetrathiafulvalene, Nafion, Biosensor, Glucose