Construction of pH-Triggered DNA Hydrogels Based on Hybridization Chain Reactions

doi:10.1007/s40242-019-0034-1

Chemical Research in Chinese Universities ›› 2020, Vol. 36 ›› Issue (2): 243-246.doi: 10.1007/s40242-019-0034-1

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Construction of pH-Triggered DNA Hydrogels Based on Hybridization Chain Reactions

LI Yujie^1,2, CHEN Jie³, DONG Yuanchen⁴, LIU Huajie², LIU Dongsheng¹

1. Key Laboratory of Organic Optoelectronics&Molecular Engineering, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China;
2. School of Chemical Science and Engineering, Shanghai Research Institute for Intelligent Autonomous Systems, Key Laboratory of Advanced Civil Engineering Materials, Ministry of Education, Tongji University, Shanghai 200092, P. R. China;
3. Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
4. CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China

Received:2019-10-31 Revised:2019-11-14 Online:2020-04-01 Published:2019-11-19
Contact: LIU Huajie, LIU Dongsheng E-mail:liuhuajie@tongji.edu.cn;liudongsheng@tsinghua.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.21890731, 21534007, 21821001, 21722310).

Abstract

Abstract: As a novel type of bio-functional material, DNA hydrogels have attracted more and more attention due to their successful applications in 3D cell culturing and tissue engineering for the designable and programmable responsiveness. Herein, we have developed a pH-triggered DNA hydrogel based on a clamped hybridization chain reaction(C-HCR). In this system, a DNA switch was designed, which can release the initiator strand in a controllable way via the formation of the C-G·C⁺ triplex under the pH stimuli. While the pre-gelation solution is stable in neutral environment, the C-HCR will trigger the sol-gel transition as the pH decreased to 5.0. This strategy has endowed the DNA hydrogel with good controllability for triggering, which also shows potential in intellectual responsiveness to certain stimuli.

Key words: DNA hydrogel, pH-Triggered, Hybridization chain reaction, Sol-gel phase transition

LI Yujie, CHEN Jie, DONG Yuanchen, LIU Huajie, LIU Dongsheng. Construction of pH-Triggered DNA Hydrogels Based on Hybridization Chain Reactions[J]. Chemical Research in Chinese Universities, 2020, 36(2): 243-246.

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Construction of pH-Triggered DNA Hydrogels Based on Hybridization Chain Reactions

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